Shalev A, Pla M, Ginsburger-Vogel T, Echalier G, Lögdberg L, Björck L, Colombani J, Segal S
J Immunol. 1983 Jan;130(1):297-302.
Serologic evidence for the existence of beta 2-microglobulin-, (beta 2m) like and H-2-like antigenic determinants in Drosophila are presented. Drosophila-cultured cells and larvae extract were shown to react specifically with a rabbit anti-rat beta 2m and a rabbit anti-mouse beta 2m antisera. G-200 pooled fractions from Drosophila larvae were shown to react with beta 2m-eluted and glycin-absorbed antisera, but not with beta 2m-absorbed or glycin-eluted antisera. These fractions also quantitatively inhibited the heterologous reaction between the anti-beta 2m antisera and purified human and rat beta 2m. The lack of reactivity of other rabbit antisera or normal serum with Drosophila, as well as the efficiency of absorption of anti-beta 2m reactivity by either rat or KCO% (Drosophila) cells, further supports the presence of beta 2m epitopes on Drosophila cells. Data are also presented showing that certain anti-H-2 alloantisera react with Drosophila. That this reactivity is indeed due to anti-H-2 antibodies is suggested by several lines of evidence, including the removal of cytotoxic alloantibodies and lack of reactivity by other mouse antisera and monoclonal reagents. Preliminary data suggest that the H-2-like and beta 2m-like determinants are physically associated on the cell surface of Drosophila cells. These findings have important implications on current concepts concerning the evolutionary origin and physiologic role of beta 2m and the major histocompatibility complex.
本文提供了果蝇中存在β2-微球蛋白（β2m）样和H-2样抗原决定簇的血清学证据。果蝇培养细胞和幼虫提取物显示与兔抗大鼠β2m和兔抗小鼠β2m抗血清发生特异性反应。果蝇幼虫的G-200汇集级分显示与β2m洗脱和甘氨酸吸收的抗血清反应，但不与β2m吸收或甘氨酸洗脱的抗血清反应。这些级分还定量抑制了抗β2m抗血清与纯化的人和大鼠β2m之间的异源反应。其他兔抗血清或正常血清与果蝇缺乏反应性，以及大鼠或KCO%（果蝇）细胞对抗β2m反应性的吸收效率，进一步支持了果蝇细胞上存在β2m表位。还提供了数据表明某些抗H-2同种异体抗血清与果蝇反应。包括细胞毒性同种异体抗体的去除以及其他小鼠抗血清和单克隆试剂缺乏反应性等几方面证据表明，这种反应性确实是由于抗H-2抗体引起的。初步数据表明，H-2样和β2m样决定簇在果蝇细胞表面物理相关。这些发现对当前关于β2m和主要组织相容性复合体的进化起源和生理作用的概念具有重要意义。
