Kazakova T B, Mel'nikova M P, Sverdlov E D
Mol Biol (Mosk). 1977 Sep-Oct;11(5):1029-38.
Fragments of rat liver mitochondrial DNA were isolated. In vivo these fragments were able to form the complexes with the proteins of inner mitochondrial membrane. The fragments represent unique DNA regions with the secondary structure, their A-T content being equal to 82%. With the aid of phosphomonoesterase, polynucleotidkinase and gamma-(32P)-ATP mtDNA fragments were labeled and analyzed for oligopyrimidine composition. It was shown that they were enriched in di- and tri-oligo-pyrimidine blocks. The fragments are shown to form in vitro a complex with the membrane proteins. A single protein m. wt. 40,000) was reisolated from the complex.
分离出大鼠肝脏线粒体DNA片段。在体内，这些片段能够与线粒体内膜的蛋白质形成复合物。这些片段代表具有二级结构的独特DNA区域，其A-T含量等于82%。借助磷酸单酯酶、多核苷酸激酶和γ-(32P)-ATP对线粒体DNA片段进行标记，并分析其寡嘧啶组成。结果表明，它们富含二聚体和三聚体寡嘧啶区段。这些片段在体外可与膜蛋白形成复合物。从复合物中重新分离出一种分子量为40000的单一蛋白质。
