通过电子显微镜定位大鼠线粒体DNA中富含A的区域并分析变性滞后现象。

Positioning of the A . T-rich regions in rat mitochondrial DNA by electron microscopy and analysis of the hysteresis of denaturation.

作者信息

Yonekawa H, Gotoh O, Motohashi J, Hayashi J I, Tagashira Y

出版信息

Biochim Biophys Acta. 1978 Dec 21;521(2):510-9. doi: 10.1016/0005-2787(78)90293-9.

Abstract

Intramolecular heterogeneity in the base composition of rat mitochondrial DNA (mtDNA) was shown by a combination of an improved denaturation mapping technique using electron microscopy and analysis of high-resolution optical melting-renaturation profiles. Circular mtDNA starts to melt in one specific region and then forms loops in four other regions in random order. These five early melting regions are all located in one half of the molecule. The arrangement of the early melting regions in rat mtDNA bears a remarkable resemblance not only to those of mtDNAs from several species of Drosophila but also to those of several species of Drosophila but also to those of several plasmid DNAs and phage DNA.

摘要

通过结合使用电子显微镜的改进变性图谱技术和高分辨率光学熔解-复性曲线分析，显示了大鼠线粒体DNA（mtDNA）碱基组成中的分子内异质性。环状mtDNA在一个特定区域开始熔解，然后在其他四个区域随机形成环。这五个早期熔解区域都位于分子的一半。大鼠mtDNA中早期熔解区域的排列不仅与几种果蝇的mtDNA非常相似，而且与几种果蝇的mtDNA以及几种质粒DNA和噬菌体DNA的排列也非常相似。