Short N J, Meek A D, Dalgarno L
J Virol. 1982 Sep;43(3):840-3. doi: 10.1128/JVI.43.3.840-843.1982.
Virus-specific polypeptide synthesis was examined in BHK cells and Vero cells infected with Bunyamwera virus. In BHK cells, in addition to the four previously reported virus-coded proteins (L, G1, G2, and N), three other infection-specific proteins were detected. These proteins, of nominal molecular weight 50,000 (p50), 16,000 (p16), and 13,000 (p13), were not labeled in mock-infected cells, were first synthesized between 4 and 8 h after infection, and were relatively prominent among the limited number of proteins generated late in infection. In preparations of purified Bunyamwera virus from BHK cell supernatants, p16 was detected but not p50 or p13. In Vero cells infected with Bunyamwera virus, both p50 and p13 were labeled strongly. Maprik virus, a member of the Mapputta group of arboviruses, is a member of the Bunyavirus genus (S.E. Newton, unpublished data). Maprik virus did not induce the synthesis of p50, p16, or p13; however, two smaller proteins (p17 and p15) which may correspond to p16 and p13 were labeled late in Maprik infection. Our data argue that p16 is a virus-coded component of the Bunyamwera virus particle and that p50 and p13 are virus-coded, nonstructural proteins.
在感染布尼亚姆韦拉病毒的BHK细胞和Vero细胞中检测了病毒特异性多肽合成。在BHK细胞中，除了先前报道的四种病毒编码蛋白（L、G1、G2和N）外，还检测到另外三种感染特异性蛋白。这些蛋白的标称分子量分别为50,000（p50）、16,000（p16）和13,000（p13），在 mock 感染的细胞中未被标记，在感染后4至8小时首次合成，并且在感染后期产生的有限数量的蛋白中相对突出。在从BHK细胞上清液中纯化的布尼亚姆韦拉病毒制剂中，检测到了p16，但未检测到p50或p13。在感染布尼亚姆韦拉病毒的Vero细胞中，p50和p13都被强烈标记。马普里克病毒是马普塔组虫媒病毒的成员，属于布尼亚病毒属（S.E.牛顿，未发表数据）。马普里克病毒未诱导p50、p16或p13的合成；然而，在马普里克病毒感染后期，两种较小的蛋白（p17和p15）可能与p16和p13相对应，被标记。我们的数据表明，p16是布尼亚姆韦拉病毒颗粒的病毒编码成分，而p50和p13是病毒编码的非结构蛋白。
