Smetana O, Eylan E, Kariv N
Med Microbiol Immunol. 1982;171(2):99-112. doi: 10.1007/BF02124917.
The inhibition of herpetic keratitis by a combination of Poly I:C with DEAE-Dextran and IUDR was studied in rabbit eyes. This treatment was administered 72 h after HSV inoculation (daily) for 3 days and continued once daily for another 2-3 days. A significant improvement was detected in rabbit eyes beginning on the first day of treatment, and there was minimal scarring. This improvement in herpetic keratitis coincided with a decrease in the virus titer isolated from rabbit eyes and an increase in interferon titer detected in rabbit tears. The cornea and trigeminal ganglia of treated and control rabbits were tested in order to prove whether this combined treatment could inhibit the migration of HSV from the cornea to the trigeminal ganglia. During the active stage of the disease, HSV was isolated in a lower titer from the cornea of the treated rabbits and from the ganglia of only half of this rabbit group. During the latent stage of the disease, no HSV was isolated from the cornea of either rabbit group, but HSV was isolated from the ganglia explants of the treated rabbits in a somewhat lower titer than from the controls. This treatment administered 3 days after the virus inoculation could not prevent the migration of HSV from the infected cornea towards the trigeminal ganglia.
在兔眼中研究了聚肌胞苷酸（Poly I:C）与二乙氨基乙基葡聚糖（DEAE - Dextran）和碘苷（IUDR）联合使用对疱疹性角膜炎的抑制作用。该治疗在单纯疱疹病毒（HSV）接种后72小时开始（每日一次），持续3天，然后继续每日一次，再持续2 - 3天。从治疗的第一天开始，兔眼就有显著改善，且瘢痕形成极少。疱疹性角膜炎的这种改善与从兔眼中分离出的病毒滴度降低以及在兔泪中检测到的干扰素滴度升高相吻合。对治疗组和对照组兔子的角膜和三叉神经节进行检测，以证明这种联合治疗是否能抑制HSV从角膜向三叉神经节的迁移。在疾病的活跃期，从治疗组兔子的角膜中分离出的HSV滴度较低，且仅在该组一半兔子的神经节中分离出HSV。在疾病的潜伏期，两组兔子的角膜中均未分离出HSV，但从治疗组兔子的神经节外植体中分离出的HSV滴度略低于对照组。在病毒接种3天后进行的这种治疗无法阻止HSV从受感染的角膜向三叉神经节迁移。
