Luque J, Delgado M D, Ferrer E, Martin M, Pinilla M, Ruiz-López M C, Sancho P, Tejero C
Rev Esp Fisiol. 1982;38 Suppl:285-9.
Experimental conditions for the partition, in dextran (Dx) polyethylene glycol (PEG) biphasic systems, of circulating red cells, reticulocytes and bone marrow cells from adult rats, as well as of erythrocytes from chicken embryos, young animals and adult chickens, have been studied. Once the partition coefficient (K) has been calculated under optimal experimental conditions, a counter-current distribution (CCD) apparatus has been used for the separation of cells in those populations, and the influence of different factors on the distribution of cells has been analyzed. An increase in the distribution efficiency of heterogeneous cells (those from the chicken and bone marrow of rats) is achieved by increasing the sedimentation time during the CCD procedure. The position of cells (previously labelled with 59Fe) along the rotor of the CCD apparatus is related to cells age, and thus allowed us to apply the CCD procedure for the assay of some enzyme activities in the separated cells.
研究了成年大鼠循环红细胞、网织红细胞和骨髓细胞以及鸡胚、幼龄动物和成年鸡红细胞在葡聚糖（Dx）-聚乙二醇（PEG）双相体系中的分配实验条件。在最佳实验条件下计算出分配系数（K）后，使用逆流分配（CCD）装置分离这些群体中的细胞，并分析了不同因素对细胞分布的影响。通过增加CCD过程中的沉降时间，可以提高异质细胞（来自鸡和大鼠骨髓的细胞）的分配效率。细胞（预先用59Fe标记）在CCD装置转子上的位置与细胞年龄有关，因此使我们能够将CCD程序应用于分离细胞中某些酶活性的测定。
