Martin M, Luque J
Br Poult Sci. 1985 Apr;26(2):163-70. doi: 10.1080/00071668508416800.
The technique of fractionating cells by a counter-current distribution procedure in dextran-poly(ethylene glycol) biphasic systems was modified by increasing the settling time and decreasing the top/bottom two-phase volume ratio. Two sub-populations of 59Fe-labelled erythrocytes, namely definitive medullar cells and primitive embryonic cells, were present in the blood of young chicks until about day 8 of age. The primitive cells were progressively replaced by definitive red cells as age increased.
通过增加沉降时间和降低上下两相体积比，改进了在葡聚糖 - 聚（乙二醇）双相系统中采用逆流分配程序对细胞进行分级分离的技术。在幼雏血液中，直到约8日龄时存在两个59Fe标记红细胞亚群，即定型髓细胞和原始胚胎细胞。随着年龄增长，原始细胞逐渐被定型红细胞取代。
