Simionescu L, Aman E
Endocrinologie. 1982 Jul-Sep;20(3):187-91.
The technique described in the instructions booklet of the radioimmunoassay Insulin kit commercially known as the ROTOP-Insulin kit (CCR) tested both in our and in other laboratories did not show the desired reproducibility. Using the main ROTOP reagents (anti-insulin serum and 125I-Insulin) in radioimmunoassay (RIA) systems with other separation techniques (double antibody, dextran-charcoal and polyethyleneglycol) we observed that these reagents are suitable for a sensitive and precise RIA system. The intra-assay variations observed were caused by the separation technique with 86% ethanol as proposed by the procedure. This work describes an insulin-RIA system modified in order to be able to use the ROTOP reagents with the polyethyleneglycol (PEG) separation technique. This variant is used for more than three years in our laboratory but was adopted also in other RIA laboratories in Romania.
商业上称为ROTOP -胰岛素试剂盒（CCR）的放射免疫分析胰岛素试剂盒说明书中描述的技术，在我们实验室和其他实验室进行测试时，均未显示出预期的可重复性。在采用其他分离技术（双抗体、葡聚糖 - 活性炭和聚乙二醇）的放射免疫分析（RIA）系统中使用主要的ROTOP试剂（抗胰岛素血清和125I -胰岛素）时，我们观察到这些试剂适用于灵敏且精确的RIA系统。所观察到的批内变异是由该方法所提议的用86%乙醇的分离技术引起的。这项工作描述了一种经过改进的胰岛素 - RIA系统，以便能够将ROTOP试剂与聚乙二醇（PEG）分离技术一起使用。这种变体在我们实验室已经使用了三年多，而且罗马尼亚的其他RIA实验室也采用了。
