Sourgens H, Winterhoff H, Kemper F H, Aenstoots F
Clin Chim Acta. 1979 Oct 1;97(2-3):179-90. doi: 10.1016/0009-8981(79)90414-5.
Polyethyleneglycol (PEG) has been recommended as a separating agent in the assay of some peptide hormones (Desbuquois, B. and Aurbach, G.D. (1971) J. Clin. Endocrinol. 33, 732) and several substances of low molecular weight (Ratcliffe, J.G. (1974) Br. Med. Bull. 30, 32). In the present study the PEG-separation technique has been modified and adapted for the assay of thyroid hormones. Separation with PEG has the advantage of being cheap, rapid and relatively non-susceptible to disturbances as compared with the charcoal and double-antibody-solid phase techniques. The influence of different buffer systems, varying pH and ionic strength, on the precipitation process with PEG also has been investigated. Of the different systems tested barbital buffer containing 0.1% human serum albumin proved to be the best, preferably in the presence of bovine gamma-globulin. In the radioimmunoassay of T3 variations in pH and ionic strength are of minor importance whereas in the radioimmunoassay of T4 the adherence to a certain pH is recommended. Barbital buffer containing 0.1% bovine serum albumin was inadequate in the T3 radioimmunoassay, while Tris and phosphate buffers did not give satisfying results for either radioimmunoassay.
聚乙二醇（PEG）已被推荐作为某些肽类激素分析中的分离剂（德布夸瓦，B.和奥尔巴赫，G.D.（1971年）《临床内分泌学杂志》33卷，第732页）以及几种低分子量物质分析中的分离剂（拉特克利夫，J.G.（1974年）《英国医学公报》30卷，第32页）。在本研究中，PEG分离技术已被改进并适用于甲状腺激素的分析。与活性炭和双抗体固相技术相比，用PEG进行分离具有成本低、速度快且相对不易受干扰的优点。还研究了不同缓冲系统、不同pH值和离子强度对PEG沉淀过程的影响。在所测试的不同系统中，含有0.1%人血清白蛋白的巴比妥缓冲液被证明是最好的，最好是在存在牛γ球蛋白的情况下。在T3的放射免疫分析中，pH值和离子强度的变化不太重要，而在T4的放射免疫分析中，建议保持一定的pH值。含有0.1%牛血清白蛋白的巴比妥缓冲液在T3放射免疫分析中不合适，而Tris缓冲液和磷酸盐缓冲液在两种放射免疫分析中都未给出令人满意的结果。
