使用来自不同恶性疟原虫菌株的抗原对各种血清学检测结果进行比较。

Comparison of various serological test results using antigens from different strains of Plasmodium falciparum.

作者信息

Tharavanij S, Tantivanich S, Chongsa-Nguan M, Prasertsiriroj V

出版信息

Southeast Asian J Trop Med Public Health. 1982 Jun;13(2):174-80.

Abstract

Suitability of different strains of Plasmodium falciparum grown continuously in vitro was compared using the indirect haemagglutination (IHA) the indirect immunofluorescent (IFA) tests and ELISA. In the tests employing soluble antigens (IHA and ELISA), there was a significant higher mean log titer of the same sera tested against different strains. Ranking of the strains in term of sensitivity for the detection of malaria antibody in people in the endemic area were G-112 = SO = CC greater than SU greater than PS in the IHA test and G-112 = SO greater than CC greater than SU greater than PS in the ELISA. The difference in the mean log titers appear to relate neither to the geographical location not the isoenzyme markers tested. There was also an apparent correlation between the results of the IHA and the IFA test but not between these two tests and ELISA.

摘要

使用间接血凝试验（IHA）、间接免疫荧光试验（IFA）和酶联免疫吸附测定（ELISA）比较了不同株恶性疟原虫在体外连续培养的适宜性。在采用可溶性抗原的试验（IHA和ELISA）中，用相同血清检测不同菌株时，平均对数滴度显著更高。在IHA试验中，就检测流行地区人群疟疾抗体的敏感性而言，菌株排名为G - 112 = SO = CC大于SU大于PS；在ELISA中，排名为G - 112 = SO大于CC大于SU大于PS。平均对数滴度的差异似乎既与地理位置无关，也与所检测的同工酶标记无关。IHA和IFA试验结果之间也存在明显相关性，但这两项试验与ELISA之间不存在相关性。