用于酶联免疫吸附测定（ELISA）的恶性疟原虫体外培养粗可溶性抗原的评估。

Evaluation of a crude soluble antigen from in-vitro culture of Plasmodium falciparum for ELISA.

作者信息

Francis V S, Gupta M M, Bhat P

出版信息

Tropenmed Parasitol. 1982 Dec;33(4):240-2.

PMID:6761915

Abstract

The present study was to evaluate the soluble antigen prepared from the in-vitro cultured P. falciparum (FCR3) as an alternative source of antigen to those obtained from in-vivo models for enzyme linked immunosorbent assay (ELISA) in serology of malaria. Results obtained on known positive and negative reference sera revealed good correlation between the ELISA and the indirect fluorescent antibody (IFA) technique (rs = 0.797; p less than 0.001). However such close correlation was not observed on six local sera from patients whose peripheral blood smear showed ring stage of P. falciparum (rs = 0.43; p greater than 0.05), though all the six sera were positive by the IFA and ELISA tests. Test was repeated to establish its reproducibility. The results indicate that antigen prepared from in vitro culture and stored in liquid nitrogen was found sensitive in ELISA for serology of malaria.

摘要

本研究旨在评估从体外培养的恶性疟原虫（FCR3）制备的可溶性抗原，作为酶联免疫吸附测定（ELISA）疟疾血清学中体内模型抗原的替代来源。已知阳性和阴性参考血清的结果显示，ELISA与间接荧光抗体（IFA）技术之间具有良好的相关性（rs = 0.797；p小于0.001）。然而，在六份外周血涂片显示恶性疟原虫环状体阶段患者的本地血清中未观察到如此密切的相关性（rs = 0.43；p大于0.05），尽管所有六份血清通过IFA和ELISA检测均为阳性。重复试验以确定其可重复性。结果表明，从体外培养并储存在液氮中的抗原在ELISA疟疾血清学中具有敏感性。