Richter R F, Schirbock M, Ambrosius H
Acta Biol Med Ger. 1982;41(6):581-90.
Tolerance in carps can be induced by intracardial injection of 10-30 mg of ultracentrifuged human gamma globulin (HGGu), with strong individual variation being observable. Out of 21 carps pretreated with 10 mg HGGu, 10 animals showed 14 days after i.p. test immunization with 1 mg HGGu a log2 anti-HGG titre of 1.55 +/- 0.60 (control group - log2 7.33 +/- 1.47), while 11 animals exhibited a log2 titre of 7.45 +/- 2.75. Low doses of HGGu (0.01-1.0 mg) led to stimulation of immune response. If 1-2 mg of a human IgG myeloma protein are applied i.p. after intracardial injection of 10 mg HGGu either simultaneously or after a longer break, then a high-titre anti-idiotypical antiserum with low activity against HGG can be obtained in single cases. The experiments have shown wide individual variation in tolerance induction which may be due also to seasonal factors.
通过心内注射10 - 30毫克超速离心的人γ球蛋白（HGGu）可诱导鲤鱼产生耐受性，且可观察到明显的个体差异。在21只经10毫克HGGu预处理的鲤鱼中，10只动物在腹腔注射1毫克HGGu进行试验免疫14天后，抗HGG滴度的log2值为1.55±0.60（对照组 - log2值为7.33±1.47），而11只动物的log2滴度为7.45±2.75。低剂量的HGGu（0.01 - 1.0毫克）会刺激免疫反应。如果在心内注射10毫克HGGu后，同时或间隔较长时间腹腔注射1 - 2毫克人IgG骨髓瘤蛋白，那么在个别情况下可获得对HGG活性较低的高滴度抗独特型抗血清。实验表明，耐受性诱导存在广泛的个体差异，这也可能归因于季节因素。
