Thorel M F, Pekàrek J, Svejcar J, Kubin M, Prochàzka B
Ann Immunol (Paris). 1982 May-Jun;133C(3):325-32.
In this study, 3 groups of 15 rabbits each were sensitized with killed Mycobacterium tuberculosis, M. kansasii or M. avium bacteria. The sensitization was performed through the footpad. Eighteen days after sensitization, cellular immunity of the rabbits was tested with total homologous and heterologous antigens by direct and indirect migration inhibition tests using spleen macrophages. The bacteria were sonicated and centrifuged at 100,000 g for 1 h. The supernatant was used as the cytoplasmic antigen. Assayed by indirect migration, the migration index (MI) for homologous antigens was highly specific compared to heterologous antigens (MI of 0,60-0,65 compared to 0,79-0,96). The patterns obtained for rabbits sensitized with M. tuberculosis or M. kansasii when assayed by direct migration in the presence of homologous antigens (MI of 0,58 and 0,61, respectively) were similar. M. avium gave a distinct pattern. The results obtained indicate that crossed activity with heterologous antigens would be due to the presence of common components in the total antigens of the mycobacteria in question. On the other hand, the lower specificity of the direct test would result from the role played by humoral factors and antibodies, which might influence simultaneously migration of the target macrophages.
在本研究中，将每组15只兔子分为3组，分别用灭活的结核分枝杆菌、堪萨斯分枝杆菌或鸟分枝杆菌进行致敏。致敏通过足垫进行。致敏18天后，通过使用脾巨噬细胞的直接和间接迁移抑制试验，用全同源和异源抗原检测兔子的细胞免疫。将细菌超声处理并在100,000 g下离心1小时。上清液用作细胞质抗原。通过间接迁移测定，与异源抗原相比，同源抗原的迁移指数（MI）具有高度特异性（MI为0.60 - 0.65，而异源抗原为0.79 - 0.96）。在用同源抗原存在下通过直接迁移测定时，用结核分枝杆菌或堪萨斯分枝杆菌致敏的兔子获得的模式相似（MI分别为0.58和0.61）。鸟分枝杆菌给出了不同的模式。获得的结果表明，与异源抗原的交叉活性是由于所讨论的分枝杆菌全抗原中存在共同成分。另一方面，直接试验较低的特异性是由于体液因子和抗体所起的作用，它们可能同时影响靶巨噬细胞的迁移。
