Maitani T, Suzuki K T
Biochem Pharmacol. 1982 Oct 1;31(19):3051-5. doi: 10.1016/0006-2952(82)90079-x.
Three dextran derivatives (dextran sulfate, DEAE-dextran and Dextran T500) were injected intraperitoneally into mice to study the induction of zinc-thionein in the liver, and the changes of essential metal levels in the liver, kidneys and spleen. The former was investigated with a high performance liquid chromatograph equipped with an atomic absorption spectrophotometer, and the latter with an inductively coupled plasma-atomic emission spectrometer. Dextran sulfate and DEAE-dextran induced a large amount of hepatic zinc-thionein dose-dependently, while Dextran T500 did not induce metallothionein substantially at the doses studied 20, 40 and 60 mg/kg body wt). The injection of dextran sulfate resulted in a significant increase of spleen weight, with a transitory increase of calcium concentration in the three tissues and a significant decrease of iron level in the spleen. In accord with the induction of zinc-thionein by dextran sulfate and DEAE-dextran, zinc concentration in the liver also increased transitionally after both injections and the time-course of the hepatic zinc level in dextran sulfate showed a close resemblance to that of the calcium one.
将三种葡聚糖衍生物（硫酸葡聚糖、二乙氨基乙基葡聚糖和葡聚糖T500）腹腔注射到小鼠体内，以研究肝脏中锌硫蛋白的诱导情况以及肝脏、肾脏和脾脏中必需金属水平的变化。前者通过配备原子吸收分光光度计的高效液相色谱仪进行研究，后者则通过电感耦合等离子体原子发射光谱仪进行研究。硫酸葡聚糖和二乙氨基乙基葡聚糖能剂量依赖性地诱导大量肝脏锌硫蛋白，而在研究的剂量（20、40和60mg/kg体重）下，葡聚糖T500基本不诱导金属硫蛋白。注射硫酸葡聚糖导致脾脏重量显著增加，三种组织中的钙浓度短暂升高，脾脏中的铁水平显著降低。与硫酸葡聚糖和二乙氨基乙基葡聚糖诱导锌硫蛋白一致，两种注射后肝脏中的锌浓度也短暂升高，硫酸葡聚糖处理后肝脏锌水平的时间进程与钙的相似。
