Ridlington J W, Winge D R, Fowler B A
Biochim Biophys Acta. 1981 Mar 5;673(2):177-83.
Rats were injected subcutaneously on two consecutive days with CdCl2, and sampled animals, killed at monthly intervals from 1 to 6 months thereafter, exhibited the presence of Cd,Zn-thionein in both the liver and kidney. At 6 months, hepatic thionein was present as the two major polymorphic forms previously demonstrated in short term Cd-injection studies. [35S]cysteine incorporation studies showed that both polymorphic forms of thionein underwent continual turnover at similar rates throughout th study. The slow hepatic and renal turnover of Cd, therefore, was not due to a highly stable form of Cd-thionein, but apparently due to an inefficient mechanism for excretion of Cd from these tissues. The Cd/Zn ratio of hepatic thionein remained relatively constant, suggesting that continual thionein induction results in a long-term hepatic trapping of Zn by thionein, but the ratio of renal thionein showed a marked increase during the course of the study.
大鼠连续两天皮下注射氯化镉，此后每月间隔处死采样动物，在1至6个月内，肝脏和肾脏中均检测到镉锌硫蛋白。6个月时，肝脏硫蛋白呈现出短期镉注射研究中先前证实的两种主要多态形式。[35S]半胱氨酸掺入研究表明，在整个研究过程中，硫蛋白的两种多态形式以相似的速率持续更新。因此，镉在肝脏和肾脏中的缓慢更新并非由于镉硫蛋白的高度稳定形式，而是显然由于这些组织中镉排泄机制效率低下。肝脏硫蛋白的镉/锌比值保持相对恒定，表明持续的硫蛋白诱导导致硫蛋白对锌的长期肝脏捕获，但在研究过程中，肾脏硫蛋白的比值显著增加。
