Pistsova T V, Chervova I A
Biull Eksp Biol Med. 1982 Sep;94(9):105-7.
In the emergency stage of heart compensatory hypertrophy induced by constriction of the abdominal aorta, quantitative autoradiographic localization of newly synthesized proteins and RNA was studied in the left ventricular myocardium of adult rats 1 and 3 hours after the injection of 3H-leucine and 3H-uridine, respectively. The animals were sacrificed 1, 5 and 10 days after the operation. The amount of the autoradiographic grains was measured separately for muscle and interstitial components of the myocardium. A substantial increase in protein synthesis as regards muscle and non-muscle components was recorded only on day 10 of the experiment. At the same time incorporation of amino acids into protein of muscle and interstitial components was found to be higher by 42 and 60%, respectively. The labeling of RNA in muscle cells was similar to that of protein. In interstitial cells, the content of labeled RNA consistently rose throughout the whole experiment.
在腹主动脉缩窄诱导的心脏代偿性肥大的急性期，分别于注射³H-亮氨酸和³H-尿苷后1小时和3小时，对成年大鼠左心室心肌中新合成的蛋白质和RNA进行了定量放射自显影定位研究。术后1、5和10天处死动物。分别测量心肌的肌肉和间质成分的放射自显影颗粒数量。仅在实验的第10天，观察到肌肉和非肌肉成分的蛋白质合成显著增加。同时发现，氨基酸掺入肌肉和间质成分蛋白质中的量分别高出42%和60%。肌肉细胞中RNA的标记情况与蛋白质相似。在间质细胞中，整个实验过程中标记RNA的含量持续上升。
