Hiernaux J R, Schroer K R, Baker P J, Rudbach J A, Bona C
Eur J Immunol. 1982 Oct;12(10):797-803. doi: 10.1002/eji.1830121002.
The monoclonal antibodies produced by a variety of hybridomas making antibody specific for E. coli 0113 lipopolysaccharide (LPS) were purified by affinity chromatography and their fine specificity studied. All reacted specifically with the polysaccharide moiety of LPS from E. coli 0113 and from Neisseria lactamica; two reacted with LPS from Pseudomonas aeruginosa and one reacted with LPS from Klebsiella pneumoniae. Polyclonal and monoclonal syngeneic and semi-syngeneic anti-idiotypic antisera were produced to study the idiotypy of LPS-specific monoclonal antibodies which express a complex cross-reactive idiotype (IdX) as well as individual idiotypes. E. coli 0113 LPS-specific antibodies produced by BALB/c mice express this IdX and the kinetics for its expression was examined using mice either primed or hyperimmunized with LPS; idiotypic maturation was observed, but we were unable to detect an auto-anti-idiotypic antibody response. This IdX was expressed on E. coli 0113 LPS-specific antibodies from all strains of mice examined, indicating that its expression is not restricted by genes linked to the IgCH locus.
通过亲和层析法纯化了多种针对大肠杆菌0113脂多糖（LPS）产生特异性抗体的杂交瘤所产生的单克隆抗体，并研究了它们的精细特异性。所有抗体都能与大肠杆菌0113和乳酸奈瑟菌的LPS多糖部分发生特异性反应；两种抗体能与铜绿假单胞菌的LPS发生反应，一种抗体能与肺炎克雷伯菌的LPS发生反应。制备了多克隆和单克隆同基因及半同基因抗独特型抗血清，以研究表达复杂交叉反应独特型（IdX）以及个体独特型的LPS特异性单克隆抗体的独特型。BALB/c小鼠产生的大肠杆菌0113 LPS特异性抗体表达这种IdX，并使用用LPS预免疫或超免疫的小鼠研究其表达动力学；观察到独特型成熟，但我们未能检测到自身抗独特型抗体反应。在所检测的所有小鼠品系的大肠杆菌0113 LPS特异性抗体上都表达了这种IdX，这表明其表达不受与IgCH基因座连锁的基因的限制。
