用特性明确的抗脂质A单克隆抗体抑制脂多糖的促有丝分裂活性。
Inhibition of lipopolysaccharide mitogenicity with characterized anti-lipid A monoclonal antibodies.
作者信息
Girard R, Chaby R
出版信息
Immunology. 1985 Nov;56(3):481-7.
Abstract
Antibodies to lipid A were raised in mice immunized with non-hydrolysed Bordetella pertussis microorganisms, coated with lipid A isolated from the same bacteria. Anti-lipid A activity of immune sera was measured by radioimmunoassay. Four hybrid cell lines that secrete antibodies directed against the hydrophobic region of B. pertussis lipopolysaccharide were produced by cell fusion between myeloma cells and spleen cells from immunized C3H/He-PAS mice. Differences were observed in the potency of the isolated monoclonal antibodies to inhibit B cell proliferation induced by lipopolysaccharide (LPS) or by lipid A, suggesting a selective recognition of effector sites present on the hydrophobic region of LPS.
摘要
用未水解的百日咳博德特氏菌微生物免疫小鼠，这些微生物包被有从同一细菌分离的脂多糖A，从而产生抗脂多糖A抗体。通过放射免疫测定法测量免疫血清的抗脂多糖A活性。通过骨髓瘤细胞与免疫的C3H/He-PAS小鼠的脾细胞之间的细胞融合，产生了四种分泌针对百日咳博德特氏菌脂多糖疏水区域的抗体的杂交细胞系。观察到分离出的单克隆抗体在抑制脂多糖（LPS）或脂多糖A诱导的B细胞增殖的效力上存在差异，这表明对脂多糖疏水区域上存在的效应位点有选择性识别。
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