用特性明确的抗脂质A单克隆抗体抑制脂多糖的促有丝分裂活性。
Inhibition of lipopolysaccharide mitogenicity with characterized anti-lipid A monoclonal antibodies.
作者信息
Girard R, Chaby R
出版信息
Immunology. 1985 Nov;56(3):481-7.
Abstract
Antibodies to lipid A were raised in mice immunized with non-hydrolysed Bordetella pertussis microorganisms, coated with lipid A isolated from the same bacteria. Anti-lipid A activity of immune sera was measured by radioimmunoassay. Four hybrid cell lines that secrete antibodies directed against the hydrophobic region of B. pertussis lipopolysaccharide were produced by cell fusion between myeloma cells and spleen cells from immunized C3H/He-PAS mice. Differences were observed in the potency of the isolated monoclonal antibodies to inhibit B cell proliferation induced by lipopolysaccharide (LPS) or by lipid A, suggesting a selective recognition of effector sites present on the hydrophobic region of LPS.
摘要
用未水解的百日咳博德特氏菌微生物免疫小鼠，这些微生物包被有从同一细菌分离的脂多糖A，从而产生抗脂多糖A抗体。通过放射免疫测定法测量免疫血清的抗脂多糖A活性。通过骨髓瘤细胞与免疫的C3H/He-PAS小鼠的脾细胞之间的细胞融合，产生了四种分泌针对百日咳博德特氏菌脂多糖疏水区域的抗体的杂交细胞系。观察到分离出的单克隆抗体在抑制脂多糖（LPS）或脂多糖A诱导的B细胞增殖的效力上存在差异，这表明对脂多糖疏水区域上存在的效应位点有选择性识别。
相似文献
1
Inhibition of lipopolysaccharide mitogenicity with characterized anti-lipid A monoclonal antibodies.用特性明确的抗脂质A单克隆抗体抑制脂多糖的促有丝分裂活性。
Immunology. 1985 Nov;56(3):481-7.
2
3
Production and characterization of monoclonal antibodies directed against Bordetella pertussis lipopolysaccharide.针对百日咳博德特氏菌脂多糖的单克隆抗体的制备与特性分析
J Clin Microbiol. 1988 Feb;26(2):188-93. doi: 10.1128/jcm.26.2.188-193.1988.
4
Development of an anti-idiotype monoclonal antibody mimicking the structure of lipopolysaccharide (LPS) inner-core determinants.一种模拟脂多糖（LPS）内核决定簇结构的抗独特型单克隆抗体的研制。
Microb Pathog. 1993 Aug;15(2):103-20. doi: 10.1006/mpat.1993.1061.
5
A comparison of specificity and biological activity of polyclonal and monoclonal antibodies raised against Salmonella minnesota R595 lipopolysaccharide.针对明尼苏达沙门氏菌R595脂多糖产生的多克隆抗体和单克隆抗体的特异性及生物活性比较。
J Med Microbiol. 1990 Feb;31(2):85-93. doi: 10.1099/00222615-31-2-85.
6
Monoclonal antibodies to salmonella lipopolysaccharide: functional analysis of anti-lipid A antibodies.抗沙门氏菌脂多糖单克隆抗体：抗脂质A抗体的功能分析
Clin Exp Immunol. 1988 Apr;72(1):157-63.
7
In vitro stimulation of immune spleen cells enhances the number of anti-lipid A-producing hybridomas.体外刺激免疫脾细胞可增加产生抗脂质A的杂交瘤数量。
J Immunol Methods. 1989 Mar 10;118(1):17-24. doi: 10.1016/0022-1759(89)90047-1.
8
Inhibition of lipopolysaccharide activation of 7OZ/3 cells by anti-lipopolysaccharide antibodies.
J Immunol. 1988 Nov 1;141(9):3208-13.
9
Antigenic determinants of lipid A analyzed with synthetic models and monoclonal antibodies.利用合成模型和单克隆抗体分析脂质A的抗原决定簇。
Biochem Biophys Res Commun. 1987 Mar 13;143(2):723-31. doi: 10.1016/0006-291x(87)91414-8.
10
Effects of lipopolysaccharide on macrophages analyzed with anti-lipid A monoclonal antibodies and polymyxin B.
Eur J Immunol. 1989 Dec;19(12):2219-25. doi: 10.1002/eji.1830191207.
引用本文的文献
1
A synthetic analog of the 3-deoxy-D-manno-2-octulosonic acid disaccharide moiety of rough-type endotoxins does not bind to mouse peritoneal macrophages and human monocytes.粗糙型内毒素的3-脱氧-D-甘露-2-辛酮糖酸二糖部分的合成类似物不与小鼠腹腔巨噬细胞和人单核细胞结合。
Infect Immun. 1993 Sep;61(9):3616-24. doi: 10.1128/iai.61.9.3616-3624.1993.
2
Immunoprophylaxis and immunotherapy of gram-negative sepsis and shock with antibodies to core glycolipids and lipid A of bacterial lipopolysaccharides.用针对细菌脂多糖核心糖脂和脂质A的抗体对革兰氏阴性菌败血症和休克进行免疫预防和免疫治疗。
Infection. 1991 Nov-Dec;19(6):383-90. doi: 10.1007/BF01726444.
3
Characterization of the epitope specificity of murine monoclonal antibodies directed against lipid A.针对脂多糖A的鼠单克隆抗体的表位特异性鉴定
Infect Immun. 1992 Jun;60(6):2201-10. doi: 10.1128/iai.60.6.2201-2210.1992.
本文引用的文献
1
Preparation of iodine-131 labelled human growth hormone of high specific activity.高比活度碘-131标记人生长激素的制备
Nature. 1962 May 5;194:495-6. doi: 10.1038/194495a0.
2
Antibodies to lipid A during urinary tract infection.
J Infect Dis. 1981 Oct;144(4):319-28. doi: 10.1093/infdis/144.4.319.
3
Determination of anti-lipid A and lipid A by enzyme immunoassay.通过酶免疫测定法测定抗脂质A和脂质A
Immunobiology. 1981;158(4):380-90. doi: 10.1016/S0171-2985(81)80008-3.
4
Modulation of the immune response to lipopolysaccharide.对脂多糖免疫反应的调节。
J Immunol. 1982 Mar;128(3):1054-8.
5
Monoclonal antibodies specific for Escherichia coli J5 lipopolysaccharide: cross-reaction with other gram-negative bacterial species.针对大肠杆菌J5脂多糖的单克隆抗体：与其他革兰氏阴性菌的交叉反应。
Infect Immun. 1984 Sep;45(3):631-6. doi: 10.1128/iai.45.3.631-636.1984.
6
Immunogenic properties of lipid A.脂多糖A的免疫原性特性。
Rev Infect Dis. 1984 Jul-Aug;6(4):546-52. doi: 10.1093/clinids/6.4.546.
7
An immunoprotective monoclonal antibody to lipopolysaccharide.一种针对脂多糖的免疫保护性单克隆抗体。
J Immunol. 1984 May;132(5):2590-2.
8
Binding of a rhodamine-labeled lipopolysaccharide to lipopolysaccharide-responder and nonresponder lymphocytes.一种罗丹明标记的脂多糖与脂多糖反应性和非反应性淋巴细胞的结合。
Cell Immunol. 1984 May;85(2):531-41. doi: 10.1016/0008-8749(84)90265-x.
9
Study of the idiotypy of lipopolysaccharide-specific polyclonal and monoclonal antibodies.脂多糖特异性多克隆抗体和单克隆抗体的独特型研究
Eur J Immunol. 1982 Oct;12(10):797-803. doi: 10.1002/eji.1830121002.
10
Monoclonal antibodies against Vibrio cholerae lipopolysaccharide.抗霍乱弧菌脂多糖的单克隆抗体。
Infect Immun. 1982 Nov;38(2):449-54. doi: 10.1128/iai.38.2.449-454.1982.
Zotero 插件