Warhol M J, Antonioli D A, Pinkus G S, Burke L, Rice R H
Hum Pathol. 1982 Dec;13(12):1095-9. doi: 10.1016/s0046-8177(82)80245-1.
Involucrin, a protein subunit of keratinocyte cross-linked envelopes, is a distinctive marker for suprabasal differentiation in stratified squamous epithelium. Immunoperoxidase staining for involucrin was used to evaluate paraffin sections of tissue obtained by colposcopically directed biopsies of infectious, metaplastic, and dysplastic lesions of the cervix and vagina. Areas of normal squamous epithelium, papillary and flat condyloma acuminatum, and mature and immature squamous metaplasia showed positive staining in 99 per cent of samples lacking significant inflammation and in 60 per cent of those with moderate or severe inflammation. In contrast, only 19 per cent of the squamous cell dysplasias, even those without much inflammation, showed positive staining, and no area with moderate or severe inflammation showed positive staining. These findings indicate that expression of involucrin is modulated by cellular pathologic features and microenvironment. We suggest that immunoperoxidase staining for involucrin may be useful in distinguishing mild dysplasia from immature metaplasia and flat condyloma in some biopsy specimens in which routine histologic examination yields an indeterminate diagnosis.
兜甲蛋白是角质形成细胞交联包膜的一种蛋白质亚基，是复层鳞状上皮基底上层分化的一个独特标志物。采用免疫过氧化物酶法对兜甲蛋白进行染色，以评估通过阴道镜引导下活检获得的宫颈和阴道感染性、化生及发育异常病变组织的石蜡切片。在无明显炎症的样本中，99%的正常鳞状上皮、乳头状和平坦型尖锐湿疣以及成熟和未成熟鳞状化生区域呈阳性染色；在有中度或重度炎症的样本中，这一比例为60%。相比之下，鳞状细胞发育异常区域，即使是炎症不明显的区域，只有19%呈阳性染色，且没有中度或重度炎症区域呈阳性染色。这些发现表明，兜甲蛋白的表达受细胞病理特征和微环境的调节。我们认为，在一些常规组织学检查诊断不明确的活检标本中，对兜甲蛋白进行免疫过氧化物酶染色可能有助于区分轻度发育异常与未成熟化生及扁平湿疣。
