Sassoon A F, Said J W, Nash G, Shintaku I P, Banks-Schlegel S
Hum Pathol. 1985 May;16(5):467-70. doi: 10.1016/s0046-8177(85)80084-8.
Immunohistochemical staining for involucrin, a cytoplasmic protein synthesized during squamous maturation, was assessed in histologic sections from hysterectomy and cone biopsy specimens from patients with cervical neoplasia. In normal and condylomatous squamous epithelium, diffuse cytoplasmic staining was seen in the suprabasal layers, with no staining of the basal cells. Staining was absent in two cases of cervical intraepithelial neoplasia (CIN), grade III, in which the lesions were composed entirely of undifferentiated cells and markedly decreased in cases involving large numbers of basal cells. In 19 of 23 cases (83 per cent) of CIN, however, focal staining for involucrin was seen in large differentiated cells in the more superficial layers, and in two cases of keratinized CIN diffuse suprabasal staining was observed. Similarly, strong staining for involucrin was present in differentiated areas in one case of microinvasive squamous cell carcinoma and in 93 per cent of cases of infiltrating squamous cell carcinoma. These findings suggest that involucrin is a marker for maturation in cervical squamous epithelial neoplasms. Patterns of immunohistochemical staining for involucrin in keratinized dysplasia and differentiated squamous carcinomas should be taken into consideration if loss of involucrin staining is used as a criterion for neoplastic transformation of cervical epithelium, as has been proposed.
对一种在鳞状上皮成熟过程中合成的细胞质蛋白——兜甲蛋白进行免疫组织化学染色，在子宫颈瘤形成患者的子宫切除标本和锥形活检标本的组织切片中进行评估。在正常和湿疣性鳞状上皮中，可见基底上层弥漫性细胞质染色，基底细胞无染色。在两例III级宫颈上皮内瘤变（CIN）中无染色，其中病变完全由未分化细胞组成，而在涉及大量基底细胞的病例中染色明显减少。然而，在23例CIN中的19例（83%）中，在较表层的大的分化细胞中可见兜甲蛋白的局灶性染色，在两例角化型CIN中观察到基底上层弥漫性染色。同样，在一例微浸润性鳞状细胞癌的分化区域以及93%的浸润性鳞状细胞癌病例中存在兜甲蛋白的强染色。这些发现表明兜甲蛋白是宫颈鳞状上皮肿瘤成熟的一个标志物。如果像有人所提议的那样，将兜甲蛋白染色缺失用作宫颈上皮肿瘤转化的标准，那么在角化发育异常和分化型鳞状细胞癌中兜甲蛋白免疫组织化学染色的模式应予以考虑。
