猪胰弹性蛋白酶对人血清载脂蛋白的蛋白水解消化作用。

Proteolytic digestion of human serum apolipoproteins by porcine pancreatic elastase.

作者信息

Maeda H, Nakamura N, Uzawa H

出版信息

J Biochem. 1982 Oct;92(4):1213-8. doi: 10.1093/oxfordjournals.jbchem.a134038.

DOI:10.1093/oxfordjournals.jbchem.a134038

Abstract

We studied the proteolytic action in vitro of free and alpha 2-macroglobulin-bound porcine pancreatic elastase [EC 3.4.21.11] on the apolipoproteins of plasma: very low density lipoprotein (VLDL), low density lipoprotein (LDL), and high density lipoprotein (HDL). Polyacrylamide gel electrophoresis, isoelectric focusing and immunodiffusion tests of elastase-treated plasma lipoproteins revealed that apolipoprotein C-II and C-III polypeptides were more susceptible to elastase in free form than plasma apolipoproteins (A-I, A-II, B, and E). Elastase bound to alpha 2-macroglobulin did not show any such activities.

摘要

我们研究了游离的以及与α2-巨球蛋白结合的猪胰弹性蛋白酶[EC 3.4.21.11]在体外对血浆载脂蛋白的蛋白水解作用：极低密度脂蛋白（VLDL）、低密度脂蛋白（LDL）和高密度脂蛋白（HDL）。对经弹性蛋白酶处理的血浆脂蛋白进行聚丙烯酰胺凝胶电泳、等电聚焦和免疫扩散试验发现，载脂蛋白C-II和C-III多肽比血浆载脂蛋白（A-I、A-II、B和E）更容易受到游离形式弹性蛋白酶的作用。与α2-巨球蛋白结合的弹性蛋白酶未显示出任何此类活性。