Wu Y J, Foor W E
J Parasitol. 1982 Dec;68(6):1048-52.
Antigenic change on the surface of immature oocytes of Ascaris occurred during passage through the oviduct. Using immunodiffusion and immunoelectrophoretic techniques we examined the possible relationship between the antigenic change of immature oocytes and the junctional fluid (JF) which is present in the fertilization chamber. From the immunodiffusion it was found that the anti-immature oocyte serum (A-I serum) had more immunoprecipitation arcs than did the anti-mature oocyte serum (A-M serum) when reacted against the junctional fluid. This indicated that A-I serum contained more immunoglobulins that reacted with junctional fluid than did the A-M serum. This result was substantiated by using immunoelectrophoretic analysis and two dimension crossed immunoelectrophoresis. Our results suggest that during the migration toward the oviduct-uterine junction the immature oocytes might shed surface proteins into the luminal fluid. Alternatively, the membrane surface of mature oocytes may be altered by the interaction with the substances present in the junctional fluid.
蛔虫未成熟卵母细胞表面的抗原变化发生在其通过输卵管的过程中。我们使用免疫扩散和免疫电泳技术，研究了未成熟卵母细胞抗原变化与受精腔内存在的结合液（JF）之间的可能关系。从免疫扩散实验发现，抗未成熟卵母细胞血清（A-I血清）与结合液反应时，比抗成熟卵母细胞血清（A-M血清）产生更多的免疫沉淀弧。这表明A-I血清中与结合液发生反应的免疫球蛋白比A-M血清更多。免疫电泳分析和二维交叉免疫电泳证实了这一结果。我们的结果表明，在向输卵管-子宫交界处迁移的过程中，未成熟卵母细胞可能会将表面蛋白释放到管腔液中。或者，成熟卵母细胞的膜表面可能会因与结合液中存在的物质相互作用而发生改变。
