Stanley H A, Langreth S G, Reese R T, Trager W
J Parasitol. 1982 Dec;68(6):1059-67.
Merozoites of Plasmodium falciparum were isolated and immunocytochemically analyzed. Mature parasites from knobby (K+) and knobless (K-) strains were incubated for 4 to 5 hr in RPMI 1640 with 10% serum and 10% RBC extract. About 12 to 14% of the merozoites released were recovered by density gradient centrifugation using Percoll. From 1 to 3 X 10(9) merozoites were obtained per collection. The merozoite preparations were contaminated with 10% residual bodies, about 0.1% infected and uninfected erythrocytes, about 0.1% RBC-free trophozoites and schizonts, and numerous small (less than 0.5 microns) membrane vesicles. Merozoites from the K+ and K- strains were morphologically and, by an indirect, ferritin-labeled antibody assay using serum from immune Aotus, antigenically indistinguishable. Although the residual body coats reacted with the immune Aotus serum, the membrane vesicles, some of which were seen to be blebbing from merozoites, did not react with this serum or a serum against erythrocytes. This paper describes a procedure that can be used to obtain large numbers of merozoites with little contamination by host erythrocytes.
恶性疟原虫裂殖子被分离出来并进行了免疫细胞化学分析。来自有突（K+）和无突（K-）株的成熟寄生虫在含有10%血清和10%红细胞提取物的RPMI 1640中孵育4至5小时。通过使用Percoll的密度梯度离心法回收了约12%至14%释放的裂殖子。每次收集可获得1至3×10⁹个裂殖子。裂殖子制剂被10%的残余体、约0.1%的感染和未感染红细胞、约0.1%的无红细胞滋养体和裂殖体以及大量小（小于0.5微米）膜泡污染。来自K+和K-株的裂殖子在形态上以及通过使用免疫夜猴血清的间接铁蛋白标记抗体试验在抗原上无法区分。尽管残余体包膜与免疫夜猴血清发生反应，但膜泡，其中一些可见从裂殖子上出芽，不与该血清或抗红细胞血清发生反应。本文描述了一种可用于获得大量受宿主红细胞污染少的裂殖子的方法。
