Atkinson C T, Aikawa M, Fujino T, Tam L Q, Hui G S, Siddiqui W A
Institute of Pathology, Case Western Reserve University, Cleveland, Ohio 44106.
J Parasitol. 1987 Dec;73(6):1235-40.
Postembedding immunoelectron microscopy, using pooled serum samples from a recent vaccination experiment involving Aotus monkeys, was used to localize immune targets in Plasmodium falciparum-infected erythrocytes and free merozoites. Serum samples from Aotus monkeys, protected completely by immunization with the P. falciparum merozoite surface coat precursor protein, identified immune targets on the surface of free and intracellular merozoites as well as the cytoplasm, plasma membrane, and parasitophorous vacuole membrane of immature schizonts. Serum samples from unprotected monkeys, which had been immunized with a complex of 143-kDa, 132-kDa, and 102-kDa polypeptides reacted specifically with the rhoptries of immature schizonts and mature merozoites.
使用来自近期涉及夜猴的疫苗接种实验的混合血清样本进行包埋后免疫电子显微镜检查，以定位恶性疟原虫感染的红细胞和游离裂殖子中的免疫靶点。用恶性疟原虫裂殖子表面包膜前体蛋白免疫完全保护的夜猴血清样本，鉴定出游离和细胞内裂殖子表面以及未成熟裂殖体的细胞质、质膜和寄生泡膜上的免疫靶点。未受保护的猴子的血清样本，这些猴子用143 kDa、132 kDa和102 kDa多肽复合物免疫，与未成熟裂殖体和成熟裂殖子的棒状体发生特异性反应。
