Koren H S, Leung K H
Mol Immunol. 1982 Oct;19(10):1341-6. doi: 10.1016/0161-5890(82)90302-9.
Our studies have shown that stimulation of human natural killer (NK) cells by poly I:C does not depend on or require monocytes. In contrast, the presence of monocytes in a mixed population of mononuclear cells stimulated by poly I:C suppresses NK activity. The suppression can be partially overcome if indomethacin (10(-6) M) is added to the culture during stimulation. Culture supernatants from poly I:C stimulated monocytes do not have detectable levels of anti-viral activity but contained appreciable amounts of PGE2. Our results offer an explanation as to how NK cells may protect themselves from suppression by PGE2. We have demonstrated that IFN-activated NK cells become resistant to PGE2-mediated suppression; moreover the suppression does not require cells other than the large granular lymphocytes, the major effector cell type for NK. Taken together, the data suggest that stimulation of NK cells is dependent on and regulated by the relative levels of interferon produced by lymphocytes and PGE2 produced by monocytes.
我们的研究表明，聚肌胞苷酸（poly I:C）对人自然杀伤（NK）细胞的刺激不依赖于单核细胞，也不需要单核细胞参与。相反，在聚肌胞苷酸刺激的单核细胞混合群体中，单核细胞的存在会抑制NK活性。如果在刺激过程中向培养物中加入吲哚美辛（10⁻⁶ M），这种抑制作用可以部分被克服。聚肌胞苷酸刺激的单核细胞培养上清液中检测不到抗病毒活性，但含有相当数量的前列腺素E2（PGE2）。我们的结果解释了NK细胞如何保护自身免受PGE2的抑制。我们已经证明，干扰素激活的NK细胞对PGE2介导的抑制产生抗性；此外，这种抑制不需要除大颗粒淋巴细胞（NK的主要效应细胞类型）以外的其他细胞。综上所述，数据表明NK细胞的刺激依赖于淋巴细胞产生的干扰素和单核细胞产生的PGE2的相对水平，并受其调节。
