Koren H S, Anderson S J, Fischer D G, Copeland C S, Jensen P J
J Immunol. 1981 Nov;127(5):2007-13.
We have found that human endogenous natural killer activity as measured in a rapid 51Cr release assay is unaffected by the presence of monocytes. Moreover, stimulation of natural killer cells by poly I:C is independent of monocytes. In contrast, the presence of monocytes in a mixed population of mononuclear cells that have been stimulated by poly I:C suppresses NK activity. The suppression is shown to be partially reversible if indomethacin (10(-6) M) is added to the cultures during stimulation. Culture supernatants of monocytes stimulated with poly I:C are shown to contain PGE1 in a radioimmunoassay, and have NK inhibitory activity comparable to that obtained with exogenous PGE1 added to NK assays at a concentration range of 10(-7) to 10(-9) M. Culture supernatants from poly I:C-stimulated monocytes do not have detectable levels of antiviral activity. In contrast, plastic nonadherent cells stimulated with poly I:C produce significant levels of interferon (100 to 200 U/ml/2 x 10(6) cells) but almost undetectable amounts of PGE. Supernatants of nonadherent cells incubated with indomethacin (10(-6) M) alone augment NK activity. Taken together, the results suggest that stimulation of mononuclear cells with poly I:C is dependent on and regulated by the relative levels of interferon produced by lymphocytes and PGE produced by monocytes.
我们发现在快速51Cr释放试验中所测定的人类内源性自然杀伤活性不受单核细胞存在的影响。此外，聚肌胞苷酸（poly I:C）对自然杀伤细胞的刺激不依赖于单核细胞。相反，在经聚肌胞苷酸刺激的单核细胞混合群体中，单核细胞的存在会抑制自然杀伤活性。如果在刺激过程中向培养物中加入吲哚美辛（10^(-6) M），则显示这种抑制作用部分可逆。在放射免疫测定中，经聚肌胞苷酸刺激的单核细胞培养上清液显示含有前列腺素E1（PGE1），并且具有与在10^(-7)至10^(-9) M浓度范围内添加到自然杀伤试验中的外源性PGE1相当的自然杀伤抑制活性。经聚肌胞苷酸刺激的单核细胞培养上清液没有可检测到的抗病毒活性水平。相反，经聚肌胞苷酸刺激的塑料非贴壁细胞产生显著水平的干扰素（100至200 U/ml/2×10^6个细胞），但几乎检测不到PGE。单独用吲哚美辛（10^(-6) M）孵育的非贴壁细胞上清液可增强自然杀伤活性。综上所述，结果表明用聚肌胞苷酸刺激单核细胞取决于淋巴细胞产生的干扰素和单核细胞产生的PGE的相对水平，并受其调节。
