Mawson J B, Gavin J B, Seelye R N, Herdson P B
Stain Technol. 1982 Sep;57(5):301-5. doi: 10.3109/10520298209066727.
Fluorescein-isothiocyanate dextran (FITC-dextran; MW approximately 70,000) was used in isolated rat hearts to compare normal vascular perfusion of ventricular myocardium with the pattern and extent of reperfusion following 60 minutes of global ischemia. Its gross distribution in frozen transverse sections through the ventricles was similar to that of sodium fluorescein. However, unlike 0.1% sodium fluorescein, 6.7% FITC-dextran has a viscosity similar to that of blood, and its much higher molecular weight prevents its diffusion beyond the ischemically injured vessels. Furthermore, staining by the alcoholic periodic acid-Schiff technique enabled tracer distribution to be confirmed microscopically and distinguished competent from incompetent vessels in paraffin embedded material.
异硫氰酸荧光素葡聚糖（FITC - 葡聚糖；分子量约70,000）被用于离体大鼠心脏，以比较心室心肌的正常血管灌注与60分钟全心缺血后再灌注的模式和范围。其在经心室的冷冻横切片中的大致分布与荧光素钠相似。然而，与0.1%的荧光素钠不同，6.7%的FITC - 葡聚糖具有与血液相似的粘度，并且其高得多的分子量阻止其扩散到缺血损伤血管之外。此外，通过乙醇过碘酸 - 希夫技术染色能够在显微镜下确认示踪剂的分布，并区分石蜡包埋材料中有功能和无功能的血管。
