Klatt E C, Lukes R J, Meyer P R
Cancer. 1983 Mar 15;51(6):1119-24. doi: 10.1002/1097-0142(19830315)51:6<1119::aid-cncr2820510624>3.0.co;2-m.
Using a pH-adjusted toluidine blue stain (pH range, 2.5-6.5), we sequentially examined the staining patterns of mast cells in tissue sections taken from patients with localized or reactive mast cell lesions and benign or malignant mastocytosis syndromes. Reactive or benign lesions stained well over the entire range of pHs and were separated from malignant mast cell proliferations which stained poorly and with greatest intensity in the less acidic range (pH greater than 3.5). Patients with disseminated mast cell lesions but without tumor masses or leukemia had a staining pattern between that of benign and malignant lesions. Basophils stained intensely at pH 2.5, and metachromasia rapidly diminished at higher pH. The use of the pH dependent toluidine blue stain may be an adjunct in recognizing patients with mast cell lesions, predicting their prognosis, and distinguishing basophils from mast cells.
使用经pH值调整的甲苯胺蓝染色剂（pH范围为2.5 - 6.5），我们依次检查了取自局限性或反应性肥大细胞病变患者以及良性或恶性肥大细胞增多症综合征患者的组织切片中肥大细胞的染色模式。反应性或良性病变在整个pH范围内染色良好，与恶性肥大细胞增殖区分开来，恶性肥大细胞增殖染色较差，且在酸性较弱的范围（pH大于3.5）染色强度最大。患有弥漫性肥大细胞病变但无肿瘤肿块或白血病的患者，其染色模式介于良性和恶性病变之间。嗜碱性粒细胞在pH 2.5时染色强烈，而异染性在较高pH时迅速减弱。使用依赖pH值的甲苯胺蓝染色剂可能有助于识别肥大细胞病变患者、预测其预后以及区分嗜碱性粒细胞和肥大细胞。
