Sawa Y, Kubo Y, Hinosaka T, Kaneko T, Tanikawa K, Sakamoto K
Hepatogastroenterology. 1982 Dec;29(6):256-8.
An affinity of horseradish peroxidase for hepatitis B surface antigen (HBsAg), irrespective of antigen-antibody conjugation, was disclosed incidentally, and a new staining method of HBsAg was established. The new technique is simple, inexpensive and available for formalin-fixed, paraffin embedded sections, and does not require fresh liver tissue. Compared with three other methods, involving Shikata's orcein staining, the conventional immunoperoxidase method, and the peroxidase-anti-peroxidase method, it showed high specificity and sensitivity, which were not lower than the other three methods. Conjugation of horseradish peroxidase with HBsAg was confirmed by affinity chromatography. Attention should be paid to the fact that there is a possibility of obtaining false positive results by immunoperoxidase methods, staining antigens other than HBsAg in HBsAg positive liver tissue because of antigen-antibody independent affinity of peroxidase for HBsAg.
偶然发现辣根过氧化物酶对乙型肝炎表面抗原（HBsAg）具有亲和力，与抗原 - 抗体结合无关，并建立了一种新的HBsAg染色方法。该新技术简单、成本低，可用于福尔马林固定、石蜡包埋切片，无需新鲜肝组织。与其他三种方法（包括Shikata地衣红染色、传统免疫过氧化物酶法和过氧化物酶 - 抗过氧化物酶法）相比，它显示出高特异性和敏感性，不低于其他三种方法。通过亲和层析证实了辣根过氧化物酶与HBsAg的结合。应注意的是，由于过氧化物酶对HBsAg存在抗原 - 抗体非依赖性亲和力，免疫过氧化物酶方法可能会在HBsAg阳性肝组织中对除HBsAg以外的抗原进行染色，从而获得假阳性结果。
