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1
GDPmannose dehydrogenase and biosynthesis of alginate-like polysaccharide in a mucoid strain of Pseudomonas aeruginosa.GDP甘露糖脱氢酶与铜绿假单胞菌黏液样菌株中类藻酸盐多糖的生物合成
J Bacteriol. 1983 Feb;153(2):1107-10. doi: 10.1128/jb.153.2.1107-1110.1983.
2
GDP-mannose dehydrogenase is the key regulatory enzyme in alginate biosynthesis in Pseudomonas aeruginosa: evidence from metabolite studies.GDP-甘露糖脱氢酶是铜绿假单胞菌藻酸盐生物合成中的关键调节酶：来自代谢物研究的证据。
Microbiology (Reading). 1994 Jul;140 ( Pt 7):1745-54. doi: 10.1099/13500872-140-7-1745.
3
A metabolic study of the activity of GDP-mannose dehydrogenase and concentrations of activated intermediates of alginate biosynthesis in Pseudomonas aeruginosa.铜绿假单胞菌中GDP-甘露糖脱氢酶活性及藻酸盐生物合成活性中间体浓度的代谢研究
J Gen Microbiol. 1993 Jan;139(1):119-27. doi: 10.1099/00221287-139-1-119.
4
Purification and characterization of guanosine diphospho-D-mannose dehydrogenase. A key enzyme in the biosynthesis of alginate by Pseudomonas aeruginosa.鸟苷二磷酸-D-甘露糖脱氢酶的纯化与特性分析。铜绿假单胞菌藻酸盐生物合成中的关键酶。
J Biol Chem. 1989 Jun 5;264(16):9380-5.
5
Characterization of guanosine diphospho-D-mannose dehydrogenase from Pseudomonas aeruginosa. Structural analysis by limited proteolysis.铜绿假单胞菌鸟苷二磷酸-D-甘露糖脱氢酶的特性。有限蛋白酶解的结构分析。
J Biol Chem. 1992 Jan 15;267(2):990-6.
6
Alginate biosynthetic enzymes in mucoid and nonmucoid Pseudomonas aeruginosa: overproduction of phosphomannose isomerase, phosphomannomutase, and GDP-mannose pyrophosphorylase by overexpression of the phosphomannose isomerase (pmi) gene.黏液型和非黏液型铜绿假单胞菌中的藻酸盐生物合成酶：通过磷酸甘露糖异构酶（pmi）基因的过表达使磷酸甘露糖异构酶、磷酸甘露糖变位酶和GDP-甘露糖焦磷酸化酶过量产生。
J Bacteriol. 1987 Jul;169(7):3224-31. doi: 10.1128/jb.169.7.3224-3231.1987.
7
Pattern of changes in the activity of enzymes of GDP-D-mannuronic acid synthesis and in the level of transcription of algA, algC and algD genes accompanying the loss and emergence of mucoidy in Pseudomonas aeruginosa.铜绿假单胞菌中伴随着黏液丧失和出现的GDP-D-甘露糖醛酸合成酶活性变化模式以及algA、algC和algD基因转录水平变化模式。
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8
Crystal structure of GDP-mannose dehydrogenase: a key enzyme of alginate biosynthesis in P. aeruginosa.GDP-甘露糖脱氢酶的晶体结构：铜绿假单胞菌中藻酸盐生物合成的关键酶
Biochemistry. 2003 Apr 29;42(16):4658-68. doi: 10.1021/bi027328k.
9
Inhibition of the GDP-d-Mannose Dehydrogenase from Using Targeted Sugar Nucleotide Probes.利用靶向糖核苷酸探针抑制 GDP-D-甘露糖脱氢酶。
ACS Chem Biol. 2020 Dec 18;15(12):3086-3092. doi: 10.1021/acschembio.0c00426. Epub 2020 Nov 25.
10
Alginate biosynthesis in mucoid recombinants of Pseudomonas aeruginosa overproducing GDP-mannose dehydrogenase.铜绿假单胞菌产过量GDP-甘露糖脱氢酶的黏液重组体中的藻酸盐生物合成。
Enzyme Microb Technol. 1991 May;13(5):385-9. doi: 10.1016/0141-0229(91)90199-k.

引用本文的文献

1
The GDP-Mannose Dehydrogenase of : An Old and New Target to Fight against Antibiotics Resistance of Mucoid Strains.[具体物种名称]的GDP-甘露糖脱氢酶：对抗黏液菌株抗生素耐药性的新旧靶点
Antibiotics (Basel). 2023 Nov 22;12(12):1649. doi: 10.3390/antibiotics12121649.
2
Microbial pathogenesis in cystic fibrosis: mucoid Pseudomonas aeruginosa and Burkholderia cepacia.囊性纤维化中的微生物致病机制：黏液型铜绿假单胞菌和洋葱伯克霍尔德菌
Microbiol Rev. 1996 Sep;60(3):539-74. doi: 10.1128/mr.60.3.539-574.1996.
3
Isolation and characterization of an alginase from mucoid strains of Pseudomonas aeruginosa.从铜绿假单胞菌黏液样菌株中分离并鉴定一种藻酸盐酶
J Bacteriol. 1984 Sep;159(3):958-64. doi: 10.1128/jb.159.3.958-964.1984.
4
Cloning and expression in Pseudomonas aeruginosa of a gene involved in the production of alginate.铜绿假单胞菌中一个参与藻酸盐产生的基因的克隆与表达
J Bacteriol. 1984 Jun;158(3):1115-21. doi: 10.1128/jb.158.3.1115-1121.1984.
5
Cloning of Escherichia coli and Pseudomonas aeruginosa phosphomannose isomerase genes and their expression in alginate-negative mutants of Pseudomonas aeruginosa.大肠杆菌和铜绿假单胞菌磷酸甘露糖异构酶基因的克隆及其在铜绿假单胞菌藻酸盐阴性突变体中的表达。
J Bacteriol. 1985 Jan;161(1):249-57. doi: 10.1128/jb.161.1.249-257.1985.
6
Alginate biosynthetic enzymes in mucoid and nonmucoid Pseudomonas aeruginosa: overproduction of phosphomannose isomerase, phosphomannomutase, and GDP-mannose pyrophosphorylase by overexpression of the phosphomannose isomerase (pmi) gene.黏液型和非黏液型铜绿假单胞菌中的藻酸盐生物合成酶：通过磷酸甘露糖异构酶（pmi）基因的过表达使磷酸甘露糖异构酶、磷酸甘露糖变位酶和GDP-甘露糖焦磷酸化酶过量产生。
J Bacteriol. 1987 Jul;169(7):3224-31. doi: 10.1128/jb.169.7.3224-3231.1987.
7
Construction and characterization of Pseudomonas aeruginosa algB mutants: role of algB in high-level production of alginate.铜绿假单胞菌algB突变体的构建与特性分析：algB在藻酸盐高水平产生中的作用
J Bacteriol. 1987 Apr;169(4):1593-602. doi: 10.1128/jb.169.4.1593-1602.1987.
8
Gene algD coding for GDPmannose dehydrogenase is transcriptionally activated in mucoid Pseudomonas aeruginosa.编码GDP甘露糖脱氢酶的基因algD在黏液型铜绿假单胞菌中被转录激活。
J Bacteriol. 1987 Jan;169(1):351-8. doi: 10.1128/jb.169.1.351-358.1987.
9
Overproduction and assay of Pseudomonas aeruginosa phosphomannose isomerase.铜绿假单胞菌磷酸甘露糖异构酶的过量生产及测定
J Bacteriol. 1986 Aug;167(2):611-5. doi: 10.1128/jb.167.2.611-615.1986.
10
Alginate synthesis by Pseudomonas aeruginosa: a key pathogenic factor in chronic pulmonary infections of cystic fibrosis patients.铜绿假单胞菌合成藻酸盐：囊性纤维化患者慢性肺部感染的关键致病因素。
Clin Microbiol Rev. 1991 Apr;4(2):191-206. doi: 10.1128/CMR.4.2.191.

本文引用的文献

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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
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Detection of sugars on paper chromatograms.纸色谱上糖的检测
Nature. 1950 Sep 9;166(4219):444-5. doi: 10.1038/166444b0.
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A gas chromatographic method for the quantitative determination of hexuronic acids in alginic acid.一种用于定量测定海藻酸中己糖醛酸的气相色谱法。
Anal Biochem. 1981 Jul 1;114(2):294-8. doi: 10.1016/0003-2697(81)90484-x.
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Phenotypic differences among clinically isolated mucoid Pseudomonas aeruginosa strains.临床分离的黏液型铜绿假单胞菌菌株之间的表型差异。
J Clin Microbiol. 1982 Oct;16(4):686-91. doi: 10.1128/jcm.16.4.686-691.1982.
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A new polysaccharide resembling alginic acid isolated from pseudomonads.一种从假单胞菌中分离出的类似海藻酸的新型多糖。
J Biol Chem. 1966 Aug 25;241(16):3845-51.
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Polyuronic acids produced by Pseudomonas aeruginosa.铜绿假单胞菌产生的聚糖醛酸。
Biochemistry. 1966 Sep;5(9):2817-22. doi: 10.1021/bi00873a006.
7
Host range and properties of the Pseudomonas aeruginosa R factor R1822.铜绿假单胞菌R因子R1822的宿主范围及特性
J Bacteriol. 1973 Feb;113(2):772-80. doi: 10.1128/jb.113.2.772-780.1973.
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Production and characterization of the slime polysaccharide of Pseudomonas aeruginosa.铜绿假单胞菌黏液多糖的产生及特性研究
J Bacteriol. 1973 Nov;116(2):915-24. doi: 10.1128/jb.116.2.915-924.1973.
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A new procedure for the preparation of GDP-[U-14C]mannose.一种制备GDP-[U-¹⁴C]甘露糖的新方法。
Anal Biochem. 1976 Aug;74(2):484-7. doi: 10.1016/0003-2697(76)90229-3.
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Biosynthesis of exopolysaccharide by Pseudomonas aeruginosa.铜绿假单胞菌胞外多糖的生物合成
J Bacteriol. 1978 May;134(2):418-22. doi: 10.1128/jb.134.2.418-422.1978.

GDP甘露糖脱氢酶与铜绿假单胞菌黏液样菌株中类藻酸盐多糖的生物合成

GDPmannose dehydrogenase and biosynthesis of alginate-like polysaccharide in a mucoid strain of Pseudomonas aeruginosa.

作者信息

Pugashetti B K, Vadas L, Prihar H S, Feingold D S

出版信息

J Bacteriol. 1983 Feb;153(2):1107-10. doi: 10.1128/jb.153.2.1107-1110.1983.

DOI:10.1128/jb.153.2.1107-1110.1983
PMID:6185463
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC221746/
Abstract

GDPmannose dehydrogenase (EC 1.1.1.132) in a mucoid strain of Pseudomonas aeruginosa isolated from a patient with cystic fibrosis was identified by demonstrating the NAD-linked formation of GDPmannuronate from GDPmannose mediated by a cell extract of the organism. Nonmucoid mutant strains did not contain GDPmannose dehydrogenase, which suggests that the enzyme is involved in the biosynthesis of alginate-like polysaccharide by P. aeruginosa.

摘要

从一名囊性纤维化患者分离出的铜绿假单胞菌黏液样菌株中的GDP甘露糖脱氢酶（EC 1.1.1.132），通过证明该生物体细胞提取物介导的由GDP甘露糖形成GDP甘露糖醛酸的NAD连接反应而得以鉴定。非黏液样突变菌株不含GDP甘露糖脱氢酶，这表明该酶参与了铜绿假单胞菌藻酸盐样多糖的生物合成。