GDP甘露糖脱氢酶与铜绿假单胞菌黏液样菌株中类藻酸盐多糖的生物合成
GDPmannose dehydrogenase and biosynthesis of alginate-like polysaccharide in a mucoid strain of Pseudomonas aeruginosa.
作者信息
Pugashetti B K, Vadas L, Prihar H S, Feingold D S
出版信息
J Bacteriol. 1983 Feb;153(2):1107-10. doi: 10.1128/jb.153.2.1107-1110.1983.
Abstract
GDPmannose dehydrogenase (EC 1.1.1.132) in a mucoid strain of Pseudomonas aeruginosa isolated from a patient with cystic fibrosis was identified by demonstrating the NAD-linked formation of GDPmannuronate from GDPmannose mediated by a cell extract of the organism. Nonmucoid mutant strains did not contain GDPmannose dehydrogenase, which suggests that the enzyme is involved in the biosynthesis of alginate-like polysaccharide by P. aeruginosa.
摘要
从一名囊性纤维化患者分离出的铜绿假单胞菌黏液样菌株中的GDP甘露糖脱氢酶（EC 1.1.1.132），通过证明该生物体细胞提取物介导的由GDP甘露糖形成GDP甘露糖醛酸的NAD连接反应而得以鉴定。非黏液样突变菌株不含GDP甘露糖脱氢酶，这表明该酶参与了铜绿假单胞菌藻酸盐样多糖的生物合成。
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