Aldrich H C, Reiskind J B
J Histochem Cytochem. 1982 Dec;30(12):1261-7. doi: 10.1177/30.12.6185561.
Myxamoebae of Physarum polycephalum are the uninucleate, haploid stage of the organism. Histochemical studies were undertaken to characterize intracellular and plasma membranes, and to provide a basis for assaying subcellular fractions for enrichment in plasma membranes. Lead salts deposition techniques were employed for hydrolytic enzymes. Alcian blue-ruthenium red, osmium tetroxide-potassium ferrocyanide, and phosphotungstic acid-chromic acid stains were evaluated for specificity for plasma membranes. Glucose 6-phosphatase was localized in endoplasmic reticulum, Golgi apparatus, and perinuclear space. 5'-Nucleotidase was localized in food vacuoles, chromatin, and plasmalemma. Acid phosphatase was in food vacuoles and Golgi apparatus. Alkaline phosphatase was in food vacuoles and endoplasmic reticulum. We conclude that none of the above enzymes is suitable as a cytochemical marker for plasma membranes of Physarum myxamoebae, but recommend instead staining ultrathin sections of membrane pellets with phosphotungstic acid-chromic acid, which stains plasma membranes selectively.
多头绒泡菌的变形体是该生物体的单核、单倍体阶段。进行了组织化学研究以表征细胞内和质膜，并为分析亚细胞组分中质膜的富集提供基础。铅盐沉积技术用于水解酶。评估了阿尔辛蓝 - 钌红、四氧化锇 - 亚铁氰化钾和磷钨酸 - 铬酸染色对质膜的特异性。葡萄糖6 - 磷酸酶定位于内质网、高尔基体和核周间隙。5'-核苷酸酶定位于食物泡、染色质和质膜。酸性磷酸酶存在于食物泡和高尔基体中。碱性磷酸酶存在于食物泡和内质网中。我们得出结论，上述酶均不适合作多头绒泡菌变形体质膜的细胞化学标记物，而是建议用磷钨酸 - 铬酸对膜沉淀的超薄切片进行染色，该染色可选择性地染质膜。
