Bernabeu C, Maziarz R, Spits H, de Vries J, Burakoff S J, Terhorst C
J Immunol. 1984 Dec;133(6):3188-94.
L cells expressing human HLA-A2 or HLA-B7 class I antigen heavy chains are not recognized by human cytotoxic T lymphocytes directed at HLA-A2 or HLA-B7 antigens. To test whether the absence of human beta 2-m was the cause of the lack of recognition by the human cytotoxic T lymphocytes, coexpression of the human beta 2-m gene and the HLA-A2 or HLA-B7 heavy chain in L cells ("double transfectants") was obtained. In addition, L cells expressing HLA-A2 or HLA-B7 antigens in association with human beta 2-m were obtained by an exchange reaction, in which human beta 2-m from serum replaced the endogenous murine beta 2-m. Both types of transfectant cells were used in 51Cr-release assays and cold target inhibition assays for human cytotoxic T cell clones which were directed at HLA-A2 or HLA-B7. Neither human CTL clones nor a mixture of CTL specific for HLA-A2 and HLA-B7 were able to recognize these cells. Several alternative explanations for these observations are discussed.
表达人HLA - A2或HLA - B7 I类抗原重链的L细胞不能被针对HLA - A2或HLA - B7抗原的人细胞毒性T淋巴细胞识别。为了检测人β2 -微球蛋白的缺失是否是导致人细胞毒性T淋巴细胞无法识别的原因，在L细胞（“双转染细胞”）中实现了人β2 -微球蛋白基因与人HLA - A2或HLA - B7重链的共表达。此外，通过交换反应获得了与人β2 -微球蛋白结合表达HLA - A2或HLA - B7抗原的L细胞，其中血清中的人β2 -微球蛋白替代了内源性小鼠β2 -微球蛋白。这两种转染细胞都用于针对HLA - A2或HLA - B7的人细胞毒性T细胞克隆的51Cr释放试验和冷靶抑制试验。人CTL克隆以及针对HLA - A2和HLA - B7的CTL混合物均无法识别这些细胞。文中讨论了对这些观察结果的几种其他解释。
