Perarnau B M, Gillet A C, Hakem R, Barad M, Lemonnier F A
Centre d'Immunologie Institut National de la Santé et de la Recherche Médicale, Marseille, France.
J Immunol. 1988 Aug 15;141(4):1383-9.
Sequential transfections of P815 murine mastocytoma cells with class I gene encoding either HLA-Cw3, HLA-A3, or HLA-B7 H chain and subsequently with a human beta 2-microglobulin gene were performed to evaluate the relative efficiency of human and murine beta 2-microglobulins in promoting the cell-surface expression of HLA-class I molecules. A 6-, 11-, and 40-fold specific enhancement of the cell-surface expression of HLA-Cw3, HLA-A3, and HLA-B7 molecules, respectively, was observed in cells co-transfected with human beta 2-microglobulin gene. This effect was attributed to a more efficient association of HLA H chains with human than with murine beta 2-microglobulin, which apparently allowed a more rapid transport of the HLA molecules from the endoplasmic reticulum to the Golgi apparatus.
对P815小鼠肥大细胞瘤细胞进行连续转染，先用编码HLA - Cw3、HLA - A3或HLA - B7重链的I类基因转染，随后用人类β2 - 微球蛋白基因转染，以评估人类和小鼠β2 - 微球蛋白在促进HLA - I类分子细胞表面表达方面的相对效率。在用人类β2 - 微球蛋白基因共转染的细胞中，分别观察到HLA - Cw3、HLA - A3和HLA - B7分子的细胞表面表达特异性增强了6倍、11倍和40倍。这种效应归因于HLA重链与人类β2 - 微球蛋白的结合比与小鼠β2 - 微球蛋白更有效，这显然使得HLA分子能更快速地从内质网转运到高尔基体。
