Ferri G L, Adrian T E, Allen J M, Soimero L, Cancellieri A, Yeats J C, Blank M, Polak J M, Bloom S R
Department of Anatomy, Tor Vergata University, Rome, Italy.
Gut. 1988 Jun;29(6):762-8. doi: 10.1136/gut.29.6.762.
The distribution of regulatory peptides was studied in the separated mucosa, submucosa and muscularis externa taken at 10 sampling sites encompassing the whole human sigmoid colon (five sites), rectum (two sites), and anal canal (three sites). Consistently high concentrations of VIP were measured in the muscle layer at most sites (proximal sigmoid: 286 (16) pmol/g, upper rectum: 269 (17), a moderate decrease being found in the distal smooth sphincter (151 (30) pmol/g). Values are expressed as mean (SE). Conversely, substance P concentrations showed an obvious decline in the recto-anal muscle (mid sigmoid: 19 (2.0) pmol/g, distal rectum: 7.1 (1.3), upper anal canal: 1.6 (0.6)). Somatostatin was mainly present in the sigmoid mucosa and submucosa (37 (9.3) and 15 (3.5) pmol/g, respectively) and showed low, but consistent concentrations in the muscle (mid sigmoid: 2.2 (0.7) pmol/g, upper anal canal: 1.5 (0.8]. Starting in the distal sigmoid colon, a distinct peak of tissue NPY was revealed, which was most striking in the muscle (of mid sigmoid: 16 (3.9) pmol/g, upper rectum: 47 (7.8), anal sphincter: 58 (14)). Peptide YY was confined to the mucosa and showed an earlier peak (upper sigmoid: 709 (186) pmol/g, mid-distal sigmoid: 1965 (484)). A clear differential distribution of regulatory peptides was thus shown in the region studied. A possible role is suggested for NPY and VIP containing nerves in the effector control of the human internal anal sphincter.
在取自涵盖整个人类乙状结肠（5个部位）、直肠（2个部位）和肛管（3个部位）的10个采样点的分离黏膜、黏膜下层和肌层中，研究了调节肽的分布情况。在大多数部位的肌层中均检测到持续高浓度的血管活性肠肽（VIP）（乙状结肠近端：286（16）pmol/g，直肠上段：269（17），在远端平滑肌括约肌中发现有适度下降（151（30）pmol/g）。数值表示为平均值（标准误）。相反，P物质浓度在直肠 - 肛管肌层中呈现明显下降（乙状结肠中部：19（2.0）pmol/g，直肠远端：7.1（1.3），肛管上段：1.6（0.6））。生长抑素主要存在于乙状结肠黏膜和黏膜下层（分别为37（9.3）和15（3.5）pmol/g），在肌层中的浓度较低但较为一致（乙状结肠中部：2.2（0.7）pmol/g，肛管上段：1.5（0.8））。从乙状结肠远端开始，发现组织神经肽Y（NPY）有一个明显的峰值，在肌层中最为显著（乙状结肠中部：16（3.9）pmol/g，直肠上段：47（7.8），肛门括约肌：58（14））。肽YY局限于黏膜，并显示出一个较早的峰值（乙状结肠上段：709（186）pmol/g，乙状结肠中 - 远端：1965（484））。因此，在所研究的区域中显示出调节肽的明显差异分布。提示含NPY和VIP的神经在人类肛门内括约肌的效应控制中可能发挥作用。
