Takemoto D J, Jilka C, Kresie R
Prep Biochem. 1982;12(4):355-75. doi: 10.1080/00327488208065682.
In this report we describe the purification and characterization of a cytostatic factor from the bitter melon (Momordica charantia). As determined by gel filtration and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), this purified factor is a single component with an apparent molecular weight of 11,000. The factor is not sensitive to boiling or to pretreatments with trypsin, ribonuclease (RNAse), or deoxyribonuclease (DNAse). As determined by radioactive precursor uptake studies, the purified factor preferentially inhibits RNA synthesis in intact tissue culture cells. Some inhibition of protein synthesis and DNA synthesis also occurs. The factor is preferentially cytostatic for IM9 human leukemic lymphocytes when compared to normal human peripheral blood lymphocytes.
在本报告中，我们描述了从苦瓜（Momordica charantia）中纯化和鉴定一种细胞生长抑制因子的过程。通过凝胶过滤和十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳（SDS - PAGE）测定，这种纯化因子是一种单一成分，表观分子量为11,000。该因子对煮沸或用胰蛋白酶、核糖核酸酶（RNA酶）或脱氧核糖核酸酶（DNA酶）预处理不敏感。通过放射性前体摄取研究确定，纯化因子优先抑制完整组织培养细胞中的RNA合成。对蛋白质合成和DNA合成也有一些抑制作用。与正常人外周血淋巴细胞相比，该因子对IM9人白血病淋巴细胞具有优先的细胞生长抑制作用。
