Takemoto D J, Dunford C, McMurray M M
Toxicon. 1982;20(3):593-9. doi: 10.1016/0041-0101(82)90053-8.
We have previously reported that a crude extract from the bitter melon (Momordica charantia) killed human leukemic lymphocytes in a dose-dependent manner while not affecting the viability of normal human lymphocyte cells at these same doses (Takemoto et al., 1980). We now report that the crude preparation has both cytostatic and cytotoxic activities which are heat stable and trypsin-sensitive. Time and dose-response curves suggest that the factors act quickly, perhaps by entry into the cell. The effects of the crude extract are complete after only 2 hr of exposure. These activities are not due to the presence of the lectins from bitter melon seeds, as these purified proteins had no activity against human lymphocytic cells.
我们之前曾报道，苦瓜（Momordica charantia）的粗提物能以剂量依赖的方式杀死人类白血病淋巴细胞，而在相同剂量下不影响正常人淋巴细胞的活力（竹本等人，1980年）。我们现在报告，该粗制制剂具有细胞生长抑制和细胞毒性活性，这些活性对热稳定且对胰蛋白酶敏感。时间和剂量反应曲线表明，这些因子作用迅速，可能是通过进入细胞发挥作用。仅暴露2小时后，粗提物的作用就已完全显现。这些活性并非源于苦瓜籽中的凝集素，因为这些纯化蛋白对人类淋巴细胞没有活性。
