Marx J A, Moore E E, Bar-Or D
Ann Emerg Med. 1983 Feb;12(2):68-70. doi: 10.1016/s0196-0644(83)80373-4.
The value of lavage alkaline phosphatase determinations following hollow visceral injury was studied. Twenty-nine mongrel dogs were subjected to partial transection of the small intestine or colon, or underwent sham laparotomy. Sequential diagnostic peritoneal lavage was performed. At one hour post injury the lavage red cell count, white cell count, and amylase levels were within normal limits in each group. The lavage alkaline phosphatase, however, was markedly elevated in both bowel-injured groups compared with the sham group. In the five-hour lavage, red cell counts and amylase levels remained normal. White cell counts were now elevated in all three groups, but the results did not distinguish the intestinal injury from the sham model. Lavage alkaline phosphatase was again significantly increased in the injured groups versus the sham model. Serum alkaline phosphatase and amylase levels were normal in all study groups at baseline and one and five hours post injury. This canine study demonstrates the utility of peritoneal lavage alkaline phosphatase levels in identifying isolated small intestine and colon injuries that were otherwise undetected by red cell, white cell, and amylase determinations.
对中空脏器损伤后灌洗碱性磷酸酶测定的价值进行了研究。29只杂种犬接受了小肠或结肠部分横断术，或进行了假剖腹手术。进行了连续的诊断性腹腔灌洗。受伤后1小时，每组的灌洗红细胞计数、白细胞计数和淀粉酶水平均在正常范围内。然而，与假手术组相比，两个肠损伤组的灌洗碱性磷酸酶明显升高。在5小时灌洗时，红细胞计数和淀粉酶水平仍保持正常。所有三组的白细胞计数现在都升高了，但结果无法区分肠损伤组和假手术模型组。与假手术模型组相比，损伤组的灌洗碱性磷酸酶再次显著升高。所有研究组在基线时以及受伤后1小时和5小时的血清碱性磷酸酶和淀粉酶水平均正常。这项犬类研究证明了腹腔灌洗碱性磷酸酶水平在识别孤立的小肠和结肠损伤方面的效用，而这些损伤通过红细胞、白细胞和淀粉酶测定无法检测到。
