Hancock J M, Sumner A T
Cytobios. 1982;35(137):37-46.
Experiments have been carried out to try and answer two questions on the role of proteins in chromosome banding: firstly, what degree of protein extraction is required before banding can be produced; and secondly, to what extent are redistribution and reorganization of chromosomal components required for the production of banding. Partial extraction of all histones, and of a group of non-histones with molecular weights mainly between 50,000 and 70,000 appears to be necessary before G-, C- or R-banding can be produced. More extensive 'dehistonization' to produce chromosome scaffolds inhibits the production of all types of bands. Protein-protein and protein-DNA cross-linking inhibits all types of banding tested, the degree of inhibition being roughly related to the degree of cross-linking, but not apparently to the type of cross-linking. The results of both sets of experiments indicate that chromosome banding of all types is dependent on the prior loss from chromosomes of a specific set of proteins, and on some alteration of the arrangement of remaining chromosomal components during the banding procedure.
其一，在能够产生显带之前需要何种程度的蛋白质提取；其二，染色体显带的产生需要染色体成分重新分布和重组到何种程度。在能够产生G带、C带或R带之前，似乎有必要对所有组蛋白以及一组分子量主要在50,000至70,000之间的非组蛋白进行部分提取。进行更广泛的“脱组蛋白作用”以产生染色体支架会抑制所有类型显带的产生。蛋白质-蛋白质和蛋白质-DNA交联会抑制所测试的所有类型的显带，抑制程度大致与交联程度相关，但显然与交联类型无关。两组实验的结果均表明，所有类型的染色体显带都依赖于染色体上一组特定蛋白质的预先缺失，以及在显带过程中剩余染色体成分排列的某种改变。
