Guialis A, Arvanitopoulou A, Patrinou-Georgoula M, Sekeris C E
FEBS Lett. 1983 Jan 10;151(1):127-33. doi: 10.1016/0014-5793(83)80357-3.
30-50 S RNP particles (monoparticles) isolated from rat liver nuclei were submitted to electrophoresis in native 0.5% agarose gels. Two RNP fractions were thus separated, a minor one remaining closer to the top of the gel (MI) and a more abundant one migrating further into the gel (MII). SDS-polyacrylamide gel electrophoresis revealed that MII contains the major monoparticle (Mr 30 000-40 000 or 'core') polypeptides and higher molecular weight proteins, whereas MI contains several minor proteins of Mr greater than 40 000. Some proteins are common to both particle classes. Urea-acrylamide gel electrophoresis revealed that HnRNA is mainly present in MII, whereas snRNA is confined to the MI particle class.
从大鼠肝细胞核中分离出的30 - 50个S RNP颗粒（单颗粒）在天然0.5%琼脂糖凝胶中进行电泳。由此分离出两个RNP组分，较小的一个留在凝胶顶部附近（MI），较多的一个向凝胶深处迁移（MII）。SDS - 聚丙烯酰胺凝胶电泳显示，MII含有主要的单颗粒（Mr 30 000 - 40 000或“核心”）多肽和更高分子量的蛋白质，而MI含有几种Mr大于40 000的次要蛋白质。有些蛋白质在两类颗粒中都有。尿素 - 丙烯酰胺凝胶电泳显示，核不均一RNA主要存在于MII中，而小核RNA局限于MI颗粒类别。
