Murata F, Tsuyama S, Suzuki S, Hamada H, Ozawa M, Muramatsu T
J Histochem Cytochem. 1983 Jan;31(1A Suppl):139-44.
Distribution of glycoconjugates in different areas of the rat kidney was studied by light and electron microscopy using six different horseradish peroxidase-labeled lectins. Glomeruli and brush borders of the proximal tubules reacted differently to these lectins, which indicated differences in the carbohydrate compositions of those regions. The ascending limb of Henle's loop (ALH) had strong binding sites for peanut agglutinin (PNA) and soybean agglutinin (SBA). Dolichos biflorus agglutinin (DBA) did not stain the cells of ALH but did stain those of distal convoluted tubules (DCT). DBA is a good marker for distinguishing ALH from DCT. DBA, PNA, and SBA were also good markers of the collecting duct. Ricinus communis agglutinin (RCA-1) and wheat germ agglutinin (WGA) diffusely stained the various components of different parts of the kidney.
使用六种不同的辣根过氧化物酶标记凝集素，通过光学显微镜和电子显微镜研究了大鼠肾脏不同区域中糖缀合物的分布。肾小球和近端小管的刷状缘对这些凝集素的反应不同，这表明这些区域的碳水化合物组成存在差异。亨氏袢升支（ALH）对花生凝集素（PNA）和大豆凝集素（SBA）有强烈的结合位点。双花扁豆凝集素（DBA）不染色ALH的细胞，但能染色远曲小管（DCT）的细胞。DBA是区分ALH和DCT的良好标记物。DBA、PNA和SBA也是集合管的良好标记物。蓖麻凝集素（RCA-1）和麦胚凝集素（WGA）对肾脏不同部位的各种成分进行弥漫性染色。
