从部分纯化的人原胶原mRNA合成全长cDNA。
Synthesis of full-length cDNAs from partially purified human procollagen mRNAs.
作者信息
Ryskov A P, Prosnyak M I, Korneev S A, Limborska S A
出版信息
Mol Biol Rep. 1982 Nov 30;8(4):213-6. doi: 10.1007/BF00776582.
PMID:6186901
Abstract
Partially purified human procollagen mRNAs have been copied using reverse transcriptase from avian myeloblastosis virus. Under standard conditions, using a high deoxynucleotide concentration, only incomplete cDNAs corresponding to one-fifth to one-half of the template could be synthesized. Addition of a ribonuclease inhibitor from human placenta in the reaction mixture allowed the synthesis of full-length cDNA copies from extremely long procollagen mRNA.
摘要
已使用来自禽成髓细胞瘤病毒的逆转录酶对部分纯化的人原胶原信使核糖核酸（mRNA）进行了复制。在标准条件下，使用高脱氧核苷酸浓度时，只能合成对应于模板五分之一至二分之一的不完整互补脱氧核糖核酸（cDNA）。在反应混合物中添加来自人胎盘的核糖核酸酶抑制剂后，能够从极长的原胶原mRNA合成全长cDNA拷贝。
相似文献
1
Synthesis of full-length cDNAs from partially purified human procollagen mRNAs.
Mol Biol Rep. 1982 Nov 30;8(4):213-6. doi: 10.1007/BF00776582.
2
Synthesis of full length cDNAs from four partially purified oviduct mRNAs.
J Biol Chem. 1978 Apr 10;253(7):2471-82.
3
Synthesis of full-length DNA copies of avian myeloblastosis virus RNA in high yields.
Proc Natl Acad Sci U S A. 1977 Jul;74(7):2840-3. doi: 10.1073/pnas.74.7.2840.
4
[Isolation and characteristics of partially purified procollagen mRNA from human fibroblasts].
Genetika. 1979 Nov;15(11):1937-43.
5
Gene specific priming of complementary DNA synthesis.
Mol Biol Rep. 1976 Nov;3(2):167-73. doi: 10.1007/BF00423231.
6
Stepwise biosynthesis in vitro of globin genes from globin mRNA by DNA polymerase of avian myeloblastosis virus.
Proc Natl Acad Sci U S A. 1976 Oct;73(10):3418-22. doi: 10.1073/pnas.73.10.3418.
7
RNA primer used in synthesis of anticomplementary DNA by reverse transcriptase of avian myeloblastosis virus.
Proc Natl Acad Sci U S A. 1980 Mar;77(3):1316-20. doi: 10.1073/pnas.77.3.1316.
8
The syntheiss of high yields of full-length reverse transcripts of globin mRNA.
Nucleic Acids Res. 1977 Oct;4(10):3455-71. doi: 10.1093/nar/4.10.3455.
9
Sequence complexity of cDNA transcribed from a diverse mRNA population.
Nucleic Acids Res. 1980 Sep 25;8(18):4259-70. doi: 10.1093/nar/8.18.4259.
10
Mechanism of linear DNA circularization: formation of 'lasso'-like structures of pre-mRNA DNA copies.
Mol Biol Rep. 1982 Apr 16;8(3):129-32. doi: 10.1007/BF00777238.
本文引用的文献
1
The collagen gene family.
Nature. 1980 Aug 14;286(5774):656-7. doi: 10.1038/286656a0.
2
Genetic disorders of collagen.
Med Biol. 1981 Feb;59(1):1-6.
3
Synthesis of a full length DNA complementary to thyroglobulin 33 S messenger RNA.
Biochem Biophys Res Commun. 1980 Apr 14;93(3):645-53. doi: 10.1016/0006-291x(80)91127-4.
4
Purification of rat liver RNase inhibitor and its effect on polyribosome integrity.
Arch Biochem Biophys. 1969 Mar;130(1):48-52. doi: 10.1016/0003-9861(69)90007-1.
Zotero 插件