从部分纯化的人原胶原mRNA合成全长cDNA。

Synthesis of full-length cDNAs from partially purified human procollagen mRNAs.

作者信息

Ryskov A P, Prosnyak M I, Korneev S A, Limborska S A

出版信息

Mol Biol Rep. 1982 Nov 30;8(4):213-6. doi: 10.1007/BF00776582.

Abstract

Partially purified human procollagen mRNAs have been copied using reverse transcriptase from avian myeloblastosis virus. Under standard conditions, using a high deoxynucleotide concentration, only incomplete cDNAs corresponding to one-fifth to one-half of the template could be synthesized. Addition of a ribonuclease inhibitor from human placenta in the reaction mixture allowed the synthesis of full-length cDNA copies from extremely long procollagen mRNA.

摘要

已使用来自禽成髓细胞瘤病毒的逆转录酶对部分纯化的人原胶原信使核糖核酸（mRNA）进行了复制。在标准条件下，使用高脱氧核苷酸浓度时，只能合成对应于模板五分之一至二分之一的不完整互补脱氧核糖核酸（cDNA）。在反应混合物中添加来自人胎盘的核糖核酸酶抑制剂后，能够从极长的原胶原mRNA合成全长cDNA拷贝。