高产合成禽成髓细胞瘤病毒RNA的全长DNA拷贝。
Synthesis of full-length DNA copies of avian myeloblastosis virus RNA in high yields.
作者信息
Myers J C, Spiegelman S, Kacian D L
出版信息
Proc Natl Acad Sci U S A. 1977 Jul;74(7):2840-3. doi: 10.1073/pnas.74.7.2840.
Abstract
The synthesis of full-length complementary DNA copies of avian myeloblastosis virus RNA is described. The cDNA is estimated by electrophoresis in agarose-methylmercury gels to have a molecular weight of 2.6 X 10(6), equivalent in size to that of the RNA template. Most (60-70%) of the reaction product is the full-length material and yields correspond to 60-70% of the input RNA.
摘要
本文描述了禽成髓细胞瘤病毒RNA全长互补DNA拷贝的合成。通过在琼脂糖-甲基汞凝胶中进行电泳估计，该互补DNA的分子量为2.6×10⁶，大小与RNA模板相当。大部分（60%-70%）反应产物是全长物质，产量相当于输入RNA的60%-70%。
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