针对具有脱氢酶活性的酶的NADPH结合区域的特异性抗体。
Antibodies specific for NADPH-binding region of enzymes possessing dehydrogenase activities.
作者信息
Katiyar S S, Porter J W
出版信息
Proc Natl Acad Sci U S A. 1983 Mar;80(5):1221-3. doi: 10.1073/pnas.80.5.1221.
Abstract
The results reported in this paper show the presence of a population of antibodies in rabbit polyclonal antiserum that recognize an antigenic site at the NADPH-binding region of enzymes possessing dehydrogenase activities. Antisera from rabbits immunized with glucose-6-phosphate dehydrogenase or fatty acid synthetase were found to inactivate the enzyme dihydrofolate reductase. The inhibitory effect of this site-specific antibody is a time- and concentration-dependent reaction. This immunoinactivation is prevented by preincubation of the enzyme with NADPH.
摘要
本文报道的结果表明，兔多克隆抗血清中存在一群抗体，它们识别具有脱氢酶活性的酶的NADPH结合区域的一个抗原位点。用葡萄糖-6-磷酸脱氢酶或脂肪酸合成酶免疫的兔抗血清能使二氢叶酸还原酶失活。这种位点特异性抗体的抑制作用是一种时间和浓度依赖性反应。用NADPH对酶进行预孵育可防止这种免疫失活。
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