Lynley A M, Dale B A
Biochim Biophys Acta. 1983 Apr 14;744(1):28-35. doi: 10.1016/0167-4838(83)90336-9.
Filaggrin is a histidine-rich, cationic protein that aggregates with keratin filaments in vitro and may function as the keratin matrix protein in the terminally differentiated cells of the epidermis. This protein has been previously isolated from rodent epidermis. In this investigation, a similar protein from human skin was identified, isolated and characterized by biochemical and immunologic techniques. Indirect immunofluorescence of human skin using antiserum to rat filaggrin gave positive immunofluorescence of keratohyalin granules and the stratum corneum. This indicated the presence of a human filaggrin in the epidermis in a localization similar to that of the rodent. The protein was isolated from human epidermis and purified by ion-exchange chromatography and preparative gel electrophoresis. The purified protein crossreacts with antibody to rat filaggrin and migrates as a doublet of molecular weight (Mr) approximately 35 000 on SDS-polyacrylamide gels. It is relatively rich in polar amino acids such as histidine, arginine, serine and glycine, but is poor in nonpolar amino acids. Unlike rodent filaggrin, the human protein contains ornithine. This protein aggregates with human keratin filaments, forming compact macrofibrils in a manner analogous to that of rodent filaggrin. Thus, a human epidermal protein has been isolated which has many of the characteristics of rodent filaggrin and may function as the human keratin matrix protein.
丝聚合蛋白是一种富含组氨酸的阳离子蛋白，在体外可与角蛋白丝聚集，在表皮终末分化细胞中可能作为角蛋白基质蛋白发挥作用。该蛋白先前已从啮齿动物表皮中分离出来。在本研究中，利用生化和免疫技术从人皮肤中鉴定、分离并表征了一种类似的蛋白。用人抗大鼠丝聚合蛋白血清对人皮肤进行间接免疫荧光检测，角质透明颗粒和角质层呈现阳性免疫荧光。这表明人表皮中存在一种人丝聚合蛋白，其定位与啮齿动物的类似。该蛋白从人表皮中分离出来，并通过离子交换色谱和制备性凝胶电泳进行纯化。纯化后的蛋白与抗大鼠丝聚合蛋白抗体发生交叉反应，在SDS-聚丙烯酰胺凝胶上以分子量约为35000的双峰形式迁移。它相对富含极性氨基酸，如组氨酸、精氨酸、丝氨酸和甘氨酸，但非极性氨基酸含量较低。与啮齿动物丝聚合蛋白不同，人丝聚合蛋白含有鸟氨酸。该蛋白与人角蛋白丝聚集，以类似于啮齿动物丝聚合蛋白的方式形成紧密的大纤维。因此，已分离出一种人表皮蛋白，它具有许多啮齿动物丝聚合蛋白的特征，可能作为人角蛋白基质蛋白发挥作用。
