Bodger M P, Izaguirre C A, Blacklock H A, Hoffbrand A V
Blood. 1983 May;61(5):1006-10.
RFB-1 is a monoclonal antibody previously shown to react with granulocyte-monocyte progenitors (CFU-GM) and immature lymphoid cells in human bone marrow. RFB-HLA-DR is a monoclonal antibody that reacts with HLA-DR (la-like) antigens. The present study shows that the bone marrow subset reactive with both RFB-1 and RFB-HLA-DR contains all the cells that give rise to mixed hematopoietic colonies (derived from CFU-GEMM; a pluripotent human progenitor cell) as well as to megakaryocytic (megakaryocyte-CFU-derived) and erythropoietic (derived from erythroid burst-forming units, BFU-E) colonies, as shown by fluorescence-activated cell sorting and complement-mediated cytotoxicity. These results indicate that CFU-GEMM, BFU-E, and megakaryocyte-CFU express RFB-1 and la-like antigens. RFB-1 antigen is also expressed on erythroid colony-forming units (CFU-E). RFB-1 and RFB-HLA-DR are useful reagents in the study of hematopoietic stem cells.
RFB-1是一种单克隆抗体，先前已证明它可与人骨髓中的粒细胞-单核细胞祖细胞（CFU-GM）和未成熟淋巴细胞发生反应。RFB-HLA-DR是一种与HLA-DR（类la）抗原发生反应的单克隆抗体。本研究表明，与RFB-1和RFB-HLA-DR均发生反应的骨髓亚群包含所有能够产生混合造血集落（源自CFU-GEMM；一种多能人类祖细胞）以及巨核细胞集落（源自巨核细胞-CFU）和红细胞集落（源自红系爆式集落形成单位，BFU-E）的细胞，荧光激活细胞分选和补体介导的细胞毒性实验证明了这一点。这些结果表明，CFU-GEMM、BFU-E和巨核细胞-CFU表达RFB-1和类la抗原。RFB-1抗原也在红系集落形成单位（CFU-E）上表达。RFB-1和RFB-HLA-DR是研究造血干细胞的有用试剂。
