Siggens K W, Wood E J
Eur J Biochem. 1983 Mar 15;131(2):353-8. doi: 10.1111/j.1432-1033.1983.tb07270.x.
The haemocyanin of Limulus polyphemus is composed of a number (possibly 10-15) of polypeptides and is believed to be synthesised in cells called cyanoblasts. In vitro translation in the rabbit reticulocyte haemolysate system and in Xenopus oocytes, of mRNA isolated from cyanoblast-containing tissue, allowed the detection of several haemocyanin polypeptides amongst the products of translation. At least seven polypeptides with molecular weights in the range 68 000-71 000 were identified by an immunological method followed by electrophoretic characterisation on two-dimensional polyacrylamide gels. Comparison of the polypeptide patterns of authentic haemocyanin, reticulocyte lysate translation products and Xenopus oocyte translation products led to the conclusion that the polypeptides are unlikely to undergo significant post-translational modification or to possess cleavable signal sequences. It is proposed that release of haemocyanin into the haemolymph in vivo may involve bursting of the cyanoblasts.
美洲鲎的血蓝蛋白由若干（可能为10 - 15个）多肽组成，据信是在称为成蓝细胞的细胞中合成的。从含成蓝细胞的组织中分离出的mRNA，在兔网织红细胞溶血产物系统和非洲爪蟾卵母细胞中进行体外翻译，使得在翻译产物中检测到了几种血蓝蛋白多肽。通过免疫方法，随后在二维聚丙烯酰胺凝胶上进行电泳表征，鉴定出至少七种分子量在68,000 - 71,000范围内的多肽。对真实血蓝蛋白、网织红细胞裂解物翻译产物和非洲爪蟾卵母细胞翻译产物的多肽图谱进行比较后得出结论，这些多肽不太可能经历显著的翻译后修饰，也不太可能拥有可切割的信号序列。有人提出，血蓝蛋白在体内释放到血淋巴中可能涉及成蓝细胞的破裂。
