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鲎血蓝蛋白信使核糖核酸的鉴定

Identification of Limulus polyphemus haemocyanin messenger RNA.

作者信息

Wood E J, Bonaventura J

出版信息

Biochem J. 1981 May 15;196(2):653-6. doi: 10.1042/bj1960653.

DOI:10.1042/bj1960653
PMID:7317005
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1163044/
Abstract

Total RNA was isolated from cyanoblast-containing tissue taken from behind the compound eye of the horseshoe crab, Limulus polyphemus. Poly(A)-containing RNA separated from this by affinity chromatography on oligo(dT)-cellulose was translated in the rabbit reticulocyte haemolysate system in the presence of L-[35S]methionine. By using an antiserum to Limulus haemocyanin, polypeptides were isolated from the translation products which had a similar mobility to the authentic Limulus haemocyanin polypeptides as judged by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis.

摘要

从鲎（美洲鲎）复眼后方采集的含蓝细胞组织中分离出总RNA。通过在寡聚（dT）-纤维素上进行亲和层析从该总RNA中分离出含聚腺苷酸（Poly(A)）的RNA，并在L-[35S]甲硫氨酸存在的情况下于兔网织红细胞溶血产物系统中进行翻译。通过使用针对鲎血蓝蛋白的抗血清，从翻译产物中分离出多肽，通过十二烷基硫酸钠/聚丙烯酰胺凝胶电泳判断，这些多肽与天然鲎血蓝蛋白多肽具有相似的迁移率。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b4f/1163044/9a48f2cf9d0b/biochemj00398-0272-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b4f/1163044/9a48f2cf9d0b/biochemj00398-0272-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b4f/1163044/9a48f2cf9d0b/biochemj00398-0272-a.jpg

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本文引用的文献

1
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J Biol Chem. 1980 Apr 10;255(7):2645-8.
2
Fragmentation of crystalline beta-haemocyanin of Helix pomatia with plasmin and trypsin. Location of the fragments in the polypeptide chain.用纤溶酶和胰蛋白酶对苹果螺的结晶β-血蓝蛋白进行片段化。片段在多肽链中的位置。
Eur J Biochem. 1980 Feb;103(3):463-70. doi: 10.1111/j.1432-1033.1980.tb05970.x.
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Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
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The cyanoblast: hemocyanin formation in Limulus polyphemus.蓝细胞：美洲鲎中血蓝蛋白的形成
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A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.一种用于检测聚丙烯酰胺凝胶中氚标记蛋白质和核酸的胶片检测方法。
Eur J Biochem. 1974 Jul 1;46(1):83-8. doi: 10.1111/j.1432-1033.1974.tb03599.x.
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Identification of hemocyanin in the cyanocytes of Carcinus maenas.在绿青蟹的血蓝细胞中血蓝蛋白的鉴定。
Experientia. 1973 Oct 15;29(10):1265-7. doi: 10.1007/BF01935109.
7
The branchial gland: a site of haemocyanin synthesis in Octopus.鳃腺：章鱼中血蓝蛋白的合成部位。
Z Zellforsch Mikrosk Anat. 1972;132(2):193-201. doi: 10.1007/BF00307010.
8
Cell-free synthesis of a prolactin precursor directed by mRNA from cultured rat pituitary cells.由培养的大鼠垂体细胞的mRNA指导的催乳素前体的无细胞合成。
J Biol Chem. 1976 May 10;251(9):2842-7.
9
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J Biol Chem. 1976 Dec 10;251(23):7644-8.
10
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Eur J Biochem. 1976 Aug 1;67(1):247-56. doi: 10.1111/j.1432-1033.1976.tb10656.x.