Pasquali J L, Urlacher A, Storck D
Eur J Immunol. 1983 Mar;13(3):197-201. doi: 10.1002/eji.1830130304.
Different human IgM rheumatoid factor (IgM RF) idiotypes have been described defined by polyclonal rabbit anti-idiotypic antibodies. These antisera do not allow clear genetic analysis of the idiotypic determinants, be they cross-reactive or private. Therefore, we tried to obtain a set of monoclonal anti-idiotypic antibodies directed against RF idiotypes. Purified IgM RF serum from a patient with classical rheumatoid arthritis was used to immunize BALB/c mice. The spleen cells were fused with Sp 2/0 Ag 14, a nonsecreting mouse myeloma cell line, and a hybrid producing monoclonal anti-idiotypic antibody was selected. The mouse antibody, an IgG1 kappa, reacts with an identical or similar determinant located on (or close to) the binding site of all tested monoclonal or polyclonal IgM RF from totally unrelated patients with Waldenströms's macroglobulinemias or rheumatoid arthritis. The monoclonal antibody also reacts with 2 rheumatoid arthritis patients' IgG RF and with a low proportion of normal polyclonal IgM without detectable RF activity. An hypothesis is proposed to explain the existence of a such highly conserved determinant on RF idiotypes.
已通过多克隆兔抗独特型抗体描述了不同的人IgM类风湿因子（IgM RF）独特型。这些抗血清无法对独特型决定簇进行清晰的遗传学分析，无论它们是交叉反应性的还是个体特异性的。因此，我们试图获得一组针对RF独特型的单克隆抗独特型抗体。使用来自一名典型类风湿关节炎患者的纯化IgM RF血清免疫BALB/c小鼠。将脾细胞与非分泌型小鼠骨髓瘤细胞系Sp 2/0 Ag 14融合，并筛选出产生单克隆抗独特型抗体的杂交细胞。该小鼠抗体为IgG1 κ，与来自患有瓦尔登斯特伦巨球蛋白血症或类风湿关节炎的完全不相关患者的所有测试单克隆或多克隆IgM RF的结合位点上（或附近）的相同或相似决定簇发生反应。该单克隆抗体还与两名类风湿关节炎患者的IgG RF以及低比例的无可检测RF活性的正常多克隆IgM发生反应。提出了一个假说来解释RF独特型上这种高度保守决定簇的存在。
