Carson J H, Nielson M L, Barbarese E
Dev Biol. 1983 Apr;96(2):485-92. doi: 10.1016/0012-1606(83)90185-9.
Developmental regulation of myelin basic protein expression in mouse brain has been examined by comparing the myelin basic protein coding potential of mRNA in vitro with the accumulation of myelin basic protein-related polypeptides in vivo. In vitro translation of mRNA isolated from mouse brain generated eight myelin basic protein-related polypeptides with apparent molecular weights of 34K, 30K, 29K, 26K, 21.5K, 18.5K, 17K, and 14K. A similar set of eight myelin basic protein-related polypeptides with corresponding molecular weights was identified in vivo when total brain proteins were analyzed by immunoblotting. Each of the myelin basic protein-related polypeptides shows a characteristic developmental profile in terms of mRNA level and rate of accumulation implying a complex developmental program of myelin basic protein gene expression with regulation and modulation at several different biosynthetic levels.
通过比较体外mRNA的髓鞘碱性蛋白编码潜能与体内髓鞘碱性蛋白相关多肽的积累情况，研究了小鼠脑中髓鞘碱性蛋白表达的发育调控。从小鼠脑分离的mRNA进行体外翻译，产生了8种髓鞘碱性蛋白相关多肽，其表观分子量分别为34K、30K、29K、26K、21.5K、18.5K、17K和14K。当通过免疫印迹分析全脑蛋白质时，在体内鉴定出了一组类似的、具有相应分子量的8种髓鞘碱性蛋白相关多肽。每种髓鞘碱性蛋白相关多肽在mRNA水平和积累速率方面都呈现出特征性的发育模式，这意味着髓鞘碱性蛋白基因表达存在一个复杂的发育程序，在几个不同的生物合成水平上受到调控和调节。
