Klibanski A, Beitins I Z, Zervas N T, McArthur J W, Ridgway E C
J Clin Endocrinol Metab. 1983 Apr;56(4):774-80. doi: 10.1210/jcem-56-4-774.
alpha-Subunit and gonadotropin responses to a LHRH infusion (0.2 micrograms/min) for 4 h were studied in eight hyperprolactinemic amenorrheic women, ages 23-40, and in five normal women in the early follicular phase of the menstrual cycle. Basal alpha-subunit and LH concentrations were comparable to normal women; however, basal FSH concentrations were significantly (P less than 0.05) lower. Peak serum alpha, LH, and FSH concentrations during the LHRH infusion were significantly higher than controls (P less than 0.01, P less than 0.05, and P less than 0.01, respectively). Gel chromatography of serum confirmed the presence of both free alpha-subunit and intact LH which had normal biological activity. Six of the women were restudied in the early follicular phase of the cycle after return of normal ovulatory function and normalization of serum PRL concentrations. During bromocriptine therapy, peak serum alpha, LH, and FSH concentrations decreased significantly (P less than 0.02, P less than 0.05, and P less than 0.001, respectively) and were comparable to control subjects. The changes in serum alpha and gonadotropin responses to the LHRH infusion during bromocriptine therapy occurred independently of the serum estradiol concentrations. Abnormalities in the regulation of alpha-subunit and gonadotropin secretion are present in hyperprolactinemia. These abnormalities reverse with bromocriptine therapy and may occur independently of changes in gonadal steroids.
在8名年龄为23 - 40岁的高泌乳素血症闭经女性以及5名处于月经周期卵泡早期的正常女性中，研究了α亚基和促性腺激素对4小时促黄体生成素释放激素（LHRH，0.2微克/分钟）输注的反应。基础α亚基和促黄体生成素（LH）浓度与正常女性相当；然而，基础促卵泡生成素（FSH）浓度显著降低（P < 0.05）。LHRH输注期间血清α、LH和FSH的峰值浓度显著高于对照组（分别为P < 0.01、P < 0.05和P < 0.01）。血清凝胶色谱法证实存在具有正常生物活性的游离α亚基和完整LH。其中6名女性在恢复正常排卵功能且血清催乳素（PRL）浓度正常化后，在月经周期的卵泡早期再次接受研究。在溴隐亭治疗期间，血清α、LH和FSH的峰值浓度显著降低（分别为P < 0.02、P < 0.05和P < 0.001），且与对照组相当。溴隐亭治疗期间血清α和促性腺激素对LHRH输注反应的变化与血清雌二醇浓度无关。高泌乳素血症存在α亚基和促性腺激素分泌调节异常。这些异常在溴隐亭治疗后逆转，且可能独立于性腺类固醇的变化而发生。
